Literature DB >> 7601859

Cellular localization of the Escherichia coli SpoT protein.

D R Gentry1, M Cashel.   

Abstract

The SpoT protein of Escherichia coli serves as a source of degradation as well as an apparent source of synthesis of (p)ppGpp. Since the subcellular localization of SpoT might be a clue to its function, we have used SpoT-specific antisera to analyze cell extracts fractionated on sucrose gradients. We find that the SpoT protein is not bound to ribosomes or to either inner or outer membrane fractions. Although the SpoT protein is found in large aggregates, its localization is probably cytosolic.

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Year:  1995        PMID： 7601859      PMCID： PMC177113          DOI： 10.1128/jb.177.13.3890-3893.1995

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Characterization of the guanosine 5'-triphosphate 3'-diphosphate and guanosine 5'-diphosphate 3'-diphosphate degradation reaction catalyzed by a specific pyrophosphorylase from Escherichia coli.

Authors:  E A Heinemeyer; D Richter
Journal:  Biochemistry       Date:  1978-12-12       Impact factor: 3.162

2.  Separation of the inner (cytoplasmic) and outer membranes of Gram-negative bacteria.

Authors:  M J Osborn; R Munson
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

3.  Localization of the stringent protein of Escherichia coli on the 50S ribosomal subunit.

Authors:  S Ramagopal; B D Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

4.  On the turnover of ppGpp in Escherichia coli.

Authors:  J Gallant; G Margason; B Finch
Journal:  J Biol Chem       Date:  1972-10-10       Impact factor: 5.157

5.  Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.

Authors:  W A Haseltine; R Block
Journal:  Proc Natl Acad Sci U S A       Date:  1973-05       Impact factor: 11.205

6.  Reversibility of the pyrophosphoryl transfer from ATP to GTP by Escherichia coli stringent factor.

Authors:  J Sy
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

7.  Regulation of RNA synthesis in Escherichia coli. III. Degradation of guanosine 5'-diphosphate 3'-diphosphate in cold-shocked cells.

Authors:  H A Raué; M Cashel
Journal:  Biochim Biophys Acta       Date:  1975-03-21

8.  In vitro degradation of guanosine tetraphosphate (ppGpp) by an enzyme associated with the ribosomal fraction from Escherichia coli.

Authors:  E A Heinemeyer; D Richter
Journal:  FEBS Lett       Date:  1977-12-15       Impact factor: 4.124

9.  In vitro degradation of guanosine 5'-diphosphate, 3'-diphosphate.

Authors:  J Sy
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Heterogeneity of membrane vesicles from Escherichia coli and their subfractionation with antibody to ATPase.

Authors:  J F Hare; K Olden; E P Kennedy
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205
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  16 in total

1.  Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.

Authors:  C Galvani; J Terry; E E Ishiguro
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

2.  The stringent response of Mycobacterium tuberculosis is required for long-term survival.

Authors:  T P Primm; S J Andersen; V Mizrahi; D Avarbock; H Rubin; C E Barry
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

3.  Salmonella enterica serovar Typhimurium BipA exhibits two distinct ribosome binding modes.

Authors:  Megan A deLivron; Victoria L Robinson
Journal:  J Bacteriol       Date:  2008-07-11       Impact factor: 3.490

4.  Borrelia burgdorferi rel is responsible for generation of guanosine-3'-diphosphate-5'-triphosphate and growth control.

Authors:  Julia V Bugrysheva; Anton V Bryksin; Henry P Godfrey; Felipe C Cabello
Journal:  Infect Immun       Date:  2005-08       Impact factor: 3.441

5.  A highly sensitive quantification method for the accumulation of alarmone ppGpp in Arabidopsis thaliana using UPLC-ESI-qMS/MS.

Authors:  Yuta Ihara; Hiroyuki Ohta; Shinji Masuda
Journal:  J Plant Res       Date:  2015-03-10       Impact factor: 2.629

6.  Starvation-induced expression of retron-Ec107 and the role of ppGpp in multicopy single-stranded DNA production.

Authors:  P J Herzer
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

7.  Expression of spoT in Borrelia burgdorferi during serum starvation.

Authors:  Marc B Concepcion; David R Nelson
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

8.  G-protein control of the ribosome-associated stress response protein SpoT.

Authors:  Mengxi Jiang; Susan M Sullivan; Patrice K Wout; Janine R Maddock
Journal:  J Bacteriol       Date:  2007-07-06       Impact factor: 3.490

Review 9.  Control of rRNA transcription in Escherichia coli.

Authors:  C Condon; C Squires; C L Squires
Journal:  Microbiol Rev       Date:  1995-12

10.  ppGpp metabolism is involved in heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  Shao-Ran Zhang; Gui-Ming Lin; Wen-Li Chen; Li Wang; Cheng-Cai Zhang
Journal:  J Bacteriol       Date:  2013-08-09       Impact factor: 3.490
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