Literature DB >> 11274135

Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.

C Galvani1, J Terry, E E Ishiguro.   

Abstract

The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.

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Year:  2001        PMID： 11274135      PMCID： PMC95192          DOI： 10.1128/JB.183.8.2700-2703.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

Review 1.  Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress.

Authors:  K Gerdes
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Isolation of relaxed-control mutants of escherichia coli K-12 which are sensitive to glucose starvation.

Authors:  R D Mosteller; S F Kwan
Journal:  Biochem Biophys Res Commun       Date:  1976-03-22       Impact factor: 3.575

4.  The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.

Authors:  Y Kohara; K Akiyama; K Isono
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

5.  Evidence that glucose starvation-sensitive mutants are altered in the relB locus.

Authors:  R D Mosteller
Journal:  J Bacteriol       Date:  1978-02       Impact factor: 3.490

6.  Localization of the stringent protein of Escherichia coli on the 50S ribosomal subunit.

Authors:  S Ramagopal; B D Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

7.  Ribosomal proteins. Protein compositions of biosynthetic precursors and artifical subparticles from ribosomal subunits in Escherichia coli K 12.

Authors:  H E Homann; K H Nierhaus
Journal:  Eur J Biochem       Date:  1971-05-28

8.  The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family.

Authors:  M Gotfredsen; K Gerdes
Journal:  Mol Microbiol       Date:  1998-08       Impact factor: 3.501

9.  Mechanism of postsegregational killing by the hok gene product of the parB system of plasmid R1 and its homology with the relF gene product of the E. coli relB operon.

Authors:  K Gerdes; F W Bech; S T Jørgensen; A Løbner-Olesen; P B Rasmussen; T Atlung; L Boe; O Karlstrom; S Molin; K von Meyenburg
Journal:  EMBO J       Date:  1986-08       Impact factor: 11.598

10.  Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.

Authors:  F W Bech; S T Jørgensen; B Diderichsen; O H Karlström
Journal:  EMBO J       Date:  1985-04       Impact factor: 11.598
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  17 in total

1.  RelE, a global inhibitor of translation, is activated during nutritional stress.

Authors:  S K Christensen; M Mikkelsen; K Pedersen; K Gerdes
Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-20       Impact factor: 11.205

2.  A novel family of Escherichia coli toxin-antitoxin gene pairs.

Authors:  Jason M Brown; Karen Joy Shaw
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

3.  Escherichia coli rnlA and rnlB compose a novel toxin-antitoxin system.

Authors:  Mitsunori Koga; Yuichi Otsuka; Sébastien Lemire; Tetsuro Yonesaki
Journal:  Genetics       Date:  2010-10-26       Impact factor: 4.562

Review 4.  Insights into the virulence of oral biofilms: discoveries from proteomics.

Authors:  Masae Kuboniwa; Gena D Tribble; Erik L Hendrickson; Atsuo Amano; Richard J Lamont; Murray Hackett
Journal:  Expert Rev Proteomics       Date:  2012-06       Impact factor: 3.940

5.  Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules.

Authors:  Min Yang; Chunhui Gao; Yi Wang; Hua Zhang; Zheng-Guo He
Journal:  PLoS One       Date:  2010-05-17       Impact factor: 3.240

6.  Synchronized switching of multiple toxin-antitoxin modules by (p)ppGpp fluctuation.

Authors:  Chengzhe Tian; Szabolcs Semsey; Namiko Mitarai
Journal:  Nucleic Acids Res       Date:  2017-08-21       Impact factor: 16.971

7.  Rapid Curtailing of the Stringent Response by Toxin-Antitoxin Module-Encoded mRNases.

Authors:  Chengzhe Tian; Mohammad Roghanian; Mikkel Girke Jørgensen; Kim Sneppen; Michael Askvad Sørensen; Kenn Gerdes; Namiko Mitarai
Journal:  J Bacteriol       Date:  2016-06-27       Impact factor: 3.490

8.  The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE.

Authors:  Cajetan Neubauer; Yong-Gui Gao; Kasper R Andersen; Christine M Dunham; Ann C Kelley; Jendrik Hentschel; Kenn Gerdes; V Ramakrishnan; Ditlev E Brodersen
Journal:  Cell       Date:  2009-12-11       Impact factor: 41.582

9.  RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB.

Authors:  Martin Overgaard; Jonas Borch; Kenn Gerdes
Journal:  J Mol Biol       Date:  2009-09-08       Impact factor: 5.469

10.  Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses.

Authors:  Mikkel Christensen-Dalsgaard; Mikkel Girke Jørgensen; Kenn Gerdes
Journal:  Mol Microbiol       Date:  2009-11-25       Impact factor: 3.501
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