Literature DB >> 4155073

Heterogeneity of membrane vesicles from Escherichia coli and their subfractionation with antibody to ATPase.

J F Hare, K Olden, E P Kennedy.   

Abstract

The energy-transducing, Mg-Ca activated ATPase (ATP phosphohydrolase, EC 3.6.1.3) of E. coli is located on the inner surface of the cytoplasmic membrane. Antibody to purified ATPase has now been used to demonstrate that membrane vesicles as ordinarily prepared by the lysozyme-EDTA method consist of two distinct populations. About half the vesicles are everted, and thus readily agglutinated by antibody to ATPase, while half are right-side out. NADH oxidase (reduced NAD:O(2) oxidoreductase EC 1.6.99.3) activity is associated almost entirely with everted vesicles, while the ability to concentrate proline is a property of the right-side out vesicles. The results explain the failure of previous workers to observe the energization of membrane vesicles by oxidation of NADH.

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Year:  1974        PMID： 4155073      PMCID： PMC433994          DOI： 10.1073/pnas.71.12.4843

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.

Authors:  M S PATTERSON; R C GREENE
Journal:  Anal Chem       Date:  1965-06       Impact factor: 6.986

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  The localization of glycerol-3-phosphate dehydrogenase in Escherichia coli.

Authors:  J H Weiner
Journal:  J Membr Biol       Date:  1974       Impact factor: 1.843

Review 4.  Transport across isolated bacterial cytoplasmic membranes.

Authors:  H R Kaback
Journal:  Biochim Biophys Acta       Date:  1972-08-04

5.  Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles.

Authors:  E M Barnes; H R Kaback
Journal:  J Biol Chem       Date:  1971-09-10       Impact factor: 5.157

6.  Coupling between energy conservation and active transport of serine in Escherichia coli.

Authors:  G van Thienen; P W Postma
Journal:  Biochim Biophys Acta       Date:  1973-10-25

7.  Energy-transducing adenosine triphosphatase from Escherichia coli: purification, properties, and inhibition by antibody.

Authors:  R L Hanson; E P Kennedy
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

8.  Orientation of membrane vesicles from Escherichia coli prepared by different procedures.

Authors:  M Futai
Journal:  J Membr Biol       Date:  1974       Impact factor: 1.843

9.  Coupling of energy to active transport of amino acids in Escherichia coli.

Authors:  R D Simoni; M K Shallenberger
Journal:  Proc Natl Acad Sci U S A       Date:  1972-09       Impact factor: 11.205

10.  Orientation of membrane vesicles from Escherichia coli as detected by freeze-cleave electron microscopy.

Authors:  K H Altendorf; L A Staehelin
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490
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  23 in total

1.  Molecular structure of membrane vesicles from Escherichia coli.

Authors:  P Owen; H R Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

Review 2.  Bacterial respiration.

Authors:  B A Haddock; C W Jones
Journal:  Bacteriol Rev       Date:  1977-03

3.  Biochemical evidence for the reversed polarity of the outer membrane of the bacterial forespore.

Authors:  B J Wilkinson; J A Deans; D J Ellar
Journal:  Biochem J       Date:  1975-12       Impact factor: 3.857

4.  Fractionation of membrane vesicles from coliphage M13-infected Escherichia coli.

Authors:  W Wickner
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490

5.  Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.

Authors:  B A Haddock; M W Kendall-Tobias
Journal:  Biochem J       Date:  1975-12       Impact factor: 3.857

Review 6.  Determination of the orientation of membrane vesicles derived from mitochondria.

Authors:  H J Harmon
Journal:  J Bioenerg Biomembr       Date:  1987-04       Impact factor: 2.945

7.  Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli.

Authors:  P Stroobant; H R Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

Review 8.  Active transport of Ca2+ in bacteria: bioenergetics and function.

Authors:  R Devés; A F Brodie
Journal:  Mol Cell Biochem       Date:  1981-04-27       Impact factor: 3.396

9.  Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13.

Authors:  K Ito; G Mandel; W Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

10.  Functional mosaicism of membrane proteins in vesicles of Escherichia coli.

Authors:  L W Adler; B P Rosen
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490
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