Literature DB >> 7524039

RT-PCR: 'background priming' during reverse transcription.

B Frech1, E Peterhans.   

Abstract

Mesh：

Substances：

Year:  1994        PMID： 7524039      PMCID： PMC331961          DOI： 10.1093/nar/22.20.4342

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


× No keyword cloud information.
  6 in total

1.  Direct RT-PCR amplification of mRNA supported on membranes.

Authors:  A Ruiz; D Bok
Journal:  Biotechniques       Date:  1993-11       Impact factor: 1.993

2.  Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase.

Authors:  W T Tse; B G Forget
Journal:  Gene       Date:  1990-04-16       Impact factor: 3.688

3.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

4.  Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.

Authors:  J Y Tso; X H Sun; T H Kao; K S Reece; R Wu
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

5.  Region-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei.

Authors:  M R Stallcup; L D Washington
Journal:  J Biol Chem       Date:  1983-03-10       Impact factor: 5.157

6.  Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus.

Authors:  M S Colett; R Larson; C Gold; D Strick; D K Anderson; A F Purchio
Journal:  Virology       Date:  1988-07       Impact factor: 3.616
  6 in total
  6 in total

1.  Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach.

Authors:  V A Efimov; O G Chakhmakhcheva; J Archdeacon; J M Fernandez; O N Fedorkin; Y L Dorokhov; J G Atabekov
Journal:  Nucleic Acids Res       Date:  2001-11-15       Impact factor: 16.971

2.  Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus.

Authors:  Jyh-Ming Tsai; Han-Ching Wang; Jiann-Horng Leu; He-Hsuan Hsiao; Andrew H-J Wang; Guang-Hsiung Kou; Chu-Fang Lo
Journal:  J Virol       Date:  2004-10       Impact factor: 5.103

Review 3.  Hypothesis: Artifacts, Including Spurious Chimeric RNAs with a Short Homologous Sequence, Caused by Consecutive Reverse Transcriptions and Endogenous Random Primers.

Authors:  Zhiyu Peng; Chengfu Yuan; Lucas Zellmer; Siqi Liu; Ningzhi Xu; D Joshua Liao
Journal:  J Cancer       Date:  2015-05-01       Impact factor: 4.207

Review 4.  It Is Imperative to Establish a Pellucid Definition of Chimeric RNA and to Clear Up a Lot of Confusion in the Relevant Research.

Authors:  Chengfu Yuan; Yaping Han; Lucas Zellmer; Wenxiu Yang; Zhizhong Guan; Wenfeng Yu; Hai Huang; D Joshua Liao
Journal:  Int J Mol Sci       Date:  2017-03-28       Impact factor: 5.923

5.  Selective ablation of 3' RNA ends and processive RTs facilitate direct cDNA sequencing of full-length host cell and viral transcripts.

Authors:  Christian M Gallardo; Anh-Viet T Nguyen; Andrew L Routh; Bruce E Torbett
Journal:  Nucleic Acids Res       Date:  2022-09-23       Impact factor: 19.160

6.  New methods as alternative or corrective measures for the pitfalls and artifacts of reverse transcription and polymerase chain reactions (RT-PCR) in cloning chimeric or antisense-accompanied RNA.

Authors:  Chengfu Yuan; Yongming Liu; Min Yang; D Joshua Liao
Journal:  RNA Biol       Date:  2013-04-16       Impact factor: 4.652
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.