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Literature DB >> 2347496

Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase.

Abstract

We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.

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Year: 1990 PMID： 2347496 DOI： 10.1016/0378-1119(90)90047-u

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

20 in total

1. Localisation and functional studies on the 5'-nucleotidase of the cattle tick Boophilus microplus.

Authors: N Liyou; S Hamilton; R Mckenna; C Elvin; P Willadsen
Journal: Exp Appl Acarol Date: 2000-03 Impact factor: 2.132

2. Development of a quadriplex polymerase chain reaction for human cytomegalovirus detection.

Authors: P Markoulatos; V Samara; N Siafakas; E Plakokefalos; N Spyrou; M L Moncany
Journal: J Clin Lab Anal Date: 1999 Impact factor: 2.352

Review 3. Molecular biology made easy. The polymerase chain reaction.

Authors: A M Clarke; N P Mapstone; P Quirke
Journal: Histochem J Date: 1992-12

4. Chimeric cDNA clones: a novel PCR artifact.

Authors: R H Brakenhoff; J G Schoenmakers; N H Lubsen
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

5. A one-tube method of reverse transcription-PCR to efficiently amplify a 3-kilobase region from the RNA polymerase gene to the poly(A) tail of small round-structured viruses (Norwalk-like viruses).

Authors: T Ando; S S Monroe; J S Noel; R I Glass
Journal: J Clin Microbiol Date: 1997-03 Impact factor: 5.948

10. Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers.

Authors: J M Whitcomb; B A Ortiz-Conde; S H Hughes
Journal: J Virol Date: 1995-10 Impact factor: 5.103

Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase.

1. Localisation and functional studies on the 5'-nucleotidase of the cattle tick Boophilus microplus.

2. Development of a quadriplex polymerase chain reaction for human cytomegalovirus detection.

Review 3. Molecular biology made easy. The polymerase chain reaction.

4. Chimeric cDNA clones: a novel PCR artifact.

5. A one-tube method of reverse transcription-PCR to efficiently amplify a 3-kilobase region from the RNA polymerase gene to the poly(A) tail of small round-structured viruses (Norwalk-like viruses).

6. RT-PCR: 'background priming' during reverse transcription.

7. Minus-strand DNA is present within murine type C ecotropic retroviruses prior to infection.

8. Specific detection of minus strand hepatitis A virus RNA by Tail-PCR following reverse transcription.

Review 9. The polymerase chain reaction in pathology.

10. Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers.