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Literature DB >> 7505291

Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

P Kirschner¹, B Springer, U Vogel, A Meier, A Wrede, M Kiekenbeck, F C Bange, E C Böttger.

Abstract

Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.

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Year: 1993 PMID： 7505291 PMCID： PMC266149 DOI： 10.1128/jcm.31.11.2882-2889.1993

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

38 in total

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179 in total

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Journal: J Clin Microbiol Date: 2002-02 Impact factor: 5.948

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Authors: Leslie Hall; Kelly A Doerr; Sherri L Wohlfiel; Glenn D Roberts
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