Literature DB >> 12624075

Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes.

Jean-Noël Dauendorffer1, Isabelle Guillemin, Alexandra Aubry, Chantal Truffot-Pernot, Wladimir Sougakoff, Vincent Jarlier, Emmanuelle Cambau.   

Abstract

The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.

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Year:  2003        PMID: 12624075      PMCID: PMC150312          DOI: 10.1128/JCM.41.3.1311-1315.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  38 in total

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