| Literature DB >> 12624075 |
Jean-Noël Dauendorffer1, Isabelle Guillemin, Alexandra Aubry, Chantal Truffot-Pernot, Wladimir Sougakoff, Vincent Jarlier, Emmanuelle Cambau.
Abstract
The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.Entities:
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Year: 2003 PMID: 12624075 PMCID: PMC150312 DOI: 10.1128/JCM.41.3.1311-1315.2003
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948