Literature DB >> 2482222

Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA.

E C Böttger1.   

Abstract

Ribosomal RNA sequences are an appealing target for bacterial classification as well as for development of group- or species-specific DNA probes. Using the polymerase chain reaction and synthetic primers, the feasibility of this gene amplification technique for rapid sequence determination of the major 16S ribosomal RNA domains from small amounts of input DNA is demonstrated. Information useful for phylogenetic classification as well as for construction of specific DNA probes may be obtained by comparison with known sequences.

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Year:  1989        PMID： 2482222     DOI： 10.1016/0378-1097(89)90386-8

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  42 in total

Review 1.  PCR in diagnosis of infection: detection of bacteria in cerebrospinal fluids.

Authors:  Yoshimasa Yamamoto
Journal:  Clin Diagn Lab Immunol       Date:  2002-05

Review 2.  Principles and applications of methods for DNA-based typing of microbial organisms.

Authors:  D M Olive; P Bean
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

3.  DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.

Authors:  Alexandra Heininger; Marlies Binder; Andreas Ellinger; Konrad Botzenhart; Klaus Unertl; Gerd Döring
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

4.  Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid.

Authors:  J J Lu; C L Perng; S Y Lee; C C Wan
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

5.  Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.

Authors:  Tim Schuurman; Richard F de Boer; Anna M D Kooistra-Smid; Anton A van Zwet
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

6.  Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

Authors:  Sven Klaschik; Lutz E Lehmann; Ansgar Raadts; Andreas Hoeft; Frank Stuber
Journal:  Mol Biotechnol       Date:  2002-11       Impact factor: 2.695

7.  Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation).

Authors:  P P Bosshard; S Abels; R Zbinden; E C Böttger; M Altwegg
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

8.  Isolation and characterization of a fucoidan-degrading marine bacterial strain and its fucoidanase.

Authors:  Takeshi Sakai; Takashi Kawai; Ikunoshin Kato
Journal:  Mar Biotechnol (NY)       Date:  2004-06-23       Impact factor: 3.619

9.  Recognition of potentially novel human disease-associated pathogens by implementation of systematic 16S rRNA gene sequencing in the diagnostic laboratory.

Authors:  Peter M Keller; Silvana K Rampini; Andrea C Büchler; Gerhard Eich; Roger M Wanner; Roberto F Speck; Erik C Böttger; Guido V Bloemberg
Journal:  J Clin Microbiol       Date:  2010-07-14       Impact factor: 5.948

10.  Phylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment.

Authors:  J P Young; H L Downer; B D Eardly
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490
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