Literature DB >> 7501483

Efficient amplification using 'megaprimer' by asymmetric polymerase chain reaction.

A K Datta1.   

Abstract

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Year:  1995        PMID： 7501483      PMCID： PMC307417          DOI： 10.1093/nar/23.21.4530

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  11 in total

1.  "Megaprimer" method of PCR: increased template concentration improves yield.

Authors:  S Barik; M S Galinski
Journal:  Biotechniques       Date:  1991-04       Impact factor: 1.993

2.  The sialyltransferase "sialylmotif" participates in binding the donor substrate CMP-NeuAc.

Authors:  A K Datta; J C Paulson
Journal:  J Biol Chem       Date:  1995-01-27       Impact factor: 5.157

3.  Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

Authors:  M Kammann; J Laufs; J Schell; B Gronenborn
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

4.  The "megaprimer" method of site-directed mutagenesis.

Authors:  G Sarkar; S S Sommer
Journal:  Biotechniques       Date:  1990-04       Impact factor: 1.993

5.  Modification of the megaprimer method of PCR mutagenesis: improved amplification of the final product.

Authors:  A Aiyar; J Leis
Journal:  Biotechniques       Date:  1993-03       Impact factor: 1.993

6.  Megaprimer method for in vitro mutagenesis using parallel templates.

Authors:  M Upender; L Raj; M Weir
Journal:  Biotechniques       Date:  1995-01       Impact factor: 1.993

7.  The signal anchor and stem regions of the beta-galactoside alpha 2,6-sialyltransferase may each act to localize the enzyme to the Golgi apparatus.

Authors:  K J Colley; E U Lee; J C Paulson
Journal:  J Biol Chem       Date:  1992-04-15       Impact factor: 5.157

8.  Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of beta-galactoside alpha 2,6-sialyltransferase.

Authors:  E U Lee; J Roth; J C Paulson
Journal:  J Biol Chem       Date:  1989-08-15       Impact factor: 5.157

9.  Both potential dolichol recognition sequences of hamster GlcNAc-1-phosphate transferase are necessary for normal enzyme function.

Authors:  A K Datta; M A Lehrman
Journal:  J Biol Chem       Date:  1993-06-15       Impact factor: 5.157

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  30 in total

1.  Asp-89: a critical residue in maintaining the oligomeric structure of sheep liver cytosolic serine hydroxymethyltransferase.

Authors:  J V Krishna Rao; J R Jagath; B Sharma; N Appaji Rao; H S Savithri
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2.  Multiple conserved segments of E1 small nucleolar RNA are involved in the formation of a ribonucleoprotein particle in frog oocytes.

Authors:  D D Ruhl; M E Pusateri; G L Eliceiri
Journal:  Biochem J       Date:  2000-06-15       Impact factor: 3.857

3.  Use of a transient assay for studying the genetic determinants of Fv1 restriction.

Authors:  M Bock; K N Bishop; G Towers; J P Stoye
Journal:  J Virol       Date:  2000-08       Impact factor: 5.103

4.  Inhibition by etomoxir of rat liver carnitine octanoyltransferase is produced through the co-ordinate interaction with two histidine residues.

Authors:  M Morillas; J Clotet; B Rubí; D Serra; J Ariño; F G Hegardt; G Asins
Journal:  Biochem J       Date:  2000-10-15       Impact factor: 3.857

5.  A novel transcription factor involved in plant defense endowed with protein phosphatase activity.

Authors:  José L Carrasco; Gema Ancillo; Esther Mayda; Pablo Vera
Journal:  EMBO J       Date:  2003-07-01       Impact factor: 11.598

6.  A novel DNA-binding motif, hallmark of a new family of plant transcription factors.

Authors:  José L Carrasco; Gema Ancillo; María José Castelló; Pablo Vera
Journal:  Plant Physiol       Date:  2005-02       Impact factor: 8.340

7.  Differences in the Enterococcus faecalis lsa locus that influence susceptibility to quinupristin-dalfopristin and clindamycin.

Authors:  Kavindra V Singh; Barbara E Murray
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

8.  Cotranscriptional recognition of human intronic box H/ACA snoRNAs occurs in a splicing-independent manner.

Authors:  Patricia Richard; Arnold M Kiss; Xavier Darzacq; Tamás Kiss
Journal:  Mol Cell Biol       Date:  2006-04       Impact factor: 4.272

9.  The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix.

Authors:  L W Hamoen; A F Van Werkhoven; J J Bijlsma; D Dubnau; G Venema
Journal:  Genes Dev       Date:  1998-05-15       Impact factor: 11.361

10.  Rapid and efficient gene splicing using megaprimer-based protocol.

Authors:  Ji-Ren Chen; Jing-Jing Lü; Hua-Fang Wang
Journal:  Mol Biotechnol       Date:  2008-06-24       Impact factor: 2.695
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