Literature DB >> 1867858

"Megaprimer" method of PCR: increased template concentration improves yield.

S Barik1, M S Galinski.   

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Year:  1991        PMID： 1867858

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


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  25 in total

1.  Molecular mapping of epitopes involved in ligand activation of the human receptor for the neuropeptide, VIP, based on hybrids with the human secretin receptor.

Authors:  B Olde; A Sabirsh; C Owman
Journal:  J Mol Neurosci       Date:  1998-10       Impact factor: 3.444

2.  Two amino acid replacements change the substrate preference of DNA mismatch glycosylase Mig.MthI from T/G to A/G.

Authors:  Yvonne N Fondufe-Mittendorf; Christine Härer; Wilfried Kramer; Hans-Joachim Fritz
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

3.  Specific treatment of autoimmunity with recombinant invariant chains in which CLIP is replaced by self-epitopes.

Authors:  F Bischof; W Wienhold; C Wirblich; G Malcherek; O Zevering; A M Kruisbeek; A Melms
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

4.  Factors affecting start site selection at the Escherichia coli fis promoter.

Authors:  Kimberly A Walker; Robert Osuna
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

5.  Phosphorylation of specific serine residues within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro.

Authors:  A M Takacs; S Barik; T Das; A K Banerjee
Journal:  J Virol       Date:  1992-10       Impact factor: 5.103

6.  Efficient amplification using 'megaprimer' by asymmetric polymerase chain reaction.

Authors:  A K Datta
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

7.  Functional determinants of the Escherichia coli fis promoter: roles of -35, -10, and transcription initiation regions in the response to stringent control and growth phase-dependent regulation.

Authors:  K A Walker; C L Atkins; R Osuna
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

8.  Deletion analysis of the fis promoter region in Escherichia coli: antagonistic effects of integration host factor and Fis.

Authors:  T S Pratt; T Steiner; L S Feldman; K A Walker; R Osuna
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

9.  A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.

Authors:  A Urban; S Neukirchen; K E Jaeger
Journal:  Nucleic Acids Res       Date:  1997-06-01       Impact factor: 16.971

10.  Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system.

Authors:  Sidonie Wicky; Heinz Schwarz; Birgit Singer-Krüger
Journal:  Mol Cell Biol       Date:  2004-09       Impact factor: 4.272
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