Literature DB >> 7479920

Lyophilization-induced reversible changes in the secondary structure of proteins.

K Griebenow1, A M Klibanov.   

Abstract

Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, as revealed by both the quantitative analysis of the second derivative spectra and the Gaussian curve fitting of the original infrared spectra. Lyophilization substantially increases the beta-sheet content and lowers the alpha-helix content of all proteins. In all but one case, proteins become more ordered upon lyophilization.

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Year:  1995        PMID： 7479920      PMCID： PMC40552          DOI： 10.1073/pnas.92.24.10969

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  47 in total

1.  Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers.

Authors:  S J Prestrelski; N Tedeschi; T Arakawa; J F Carpenter
Journal:  Biophys J       Date:  1993-08       Impact factor: 4.033

2.  Aggregation of a lyophilized pharmaceutical protein, recombinant human albumin: effect of moisture and stabilization by excipients.

Authors:  H R Costantino; R Langer; A M Klibanov
Journal:  Biotechnology (N Y)       Date:  1995-05

3.  Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation.

Authors:  S T Freer; J Kraut; J D Robertus; H T Wright; N H Xuong
Journal:  Biochemistry       Date:  1970-04-28       Impact factor: 3.162

4.  Infrared spectroscopic studies of lyophilization- and temperature-induced protein aggregation.

Authors:  A Dong; S J Prestrelski; S D Allison; J F Carpenter
Journal:  J Pharm Sci       Date:  1995-04       Impact factor: 3.534

5.  Secondary structure characterization of microparticulate insulin powders.

Authors:  S D Yeo; P G Debendetti; S Y Patro; T M Przybycien
Journal:  J Pharm Sci       Date:  1994-12       Impact factor: 3.534

6.  Crystal and solution structures of superantigenic staphylococcal enterotoxins compared.

Authors:  B R Singh; F N Fu; D N Ledoux
Journal:  Nat Struct Biol       Date:  1994-06

7.  Secondary structure characterization of beta-lactamase inclusion bodies.

Authors:  T M Przybycien; J P Dunn; P Valax; G Georgiou
Journal:  Protein Eng       Date:  1994-01

8.  FT-IR and near-infrared FT-Raman studies of the secondary structure of insulinotropin in the solid state: alpha-helix to beta-sheet conversion induced by phenol and/or by high shear force.

Authors:  Y Kim; C A Rose; Y Liu; Y Ozaki; G Datta; A T Tu
Journal:  J Pharm Sci       Date:  1994-08       Impact factor: 3.534

Review 9.  Solid-phase aggregation of proteins under pharmaceutically relevant conditions.

Authors:  H R Costantino; R Langer; A M Klibanov
Journal:  J Pharm Sci       Date:  1994-12       Impact factor: 3.534

10.  Separation of freezing- and drying-induced denaturation of lyophilized proteins using stress-specific stabilization. II. Structural studies using infrared spectroscopy.

Authors:  S J Prestrelski; T Arakawa; J F Carpenter
Journal:  Arch Biochem Biophys       Date:  1993-06       Impact factor: 4.013
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  44 in total

1.  X-ray crystal structures of a severely desiccated protein.

Authors:  J A Bell
Journal:  Protein Sci       Date:  1999-10       Impact factor: 6.725

2.  Nativelike enzyme properties are important for optimum activity in neat organic solvents.

Authors:  K Griebenow; M Vidal; C Baéz; A M Santos; G Barletta
Journal:  J Am Chem Soc       Date:  2001-06-06       Impact factor: 15.419

3.  Protective mechanism of stabilizing excipients against dehydration in the freeze-drying of proteins.

Authors:  Yong-Hong Liao; Marc B Brown; Abdul Quader; Gary P Martin
Journal:  Pharm Res       Date:  2002-12       Impact factor: 4.200

4.  Detection of ligand- and solvent-induced shape alterations of cell-growth-regulatory human lectin galectin-1 in solution by small angle neutron and x-ray scattering.

Authors:  Lizhong He; Sabine André; Hans-Christian Siebert; Heike Helmholz; Bernd Niemeyer; Hans-Joachim Gabius
Journal:  Biophys J       Date:  2003-07       Impact factor: 4.033

5.  Critical evaluation of gamma-irradiated serum used as feeder in the culture and demonstration of putative nanobacteria and calcifying nanoparticles.

Authors:  Jan Martel; Cheng-Yeu Wu; John D Young
Journal:  PLoS One       Date:  2010-04-26       Impact factor: 3.240

6.  FTIR and nDSC as analytical tools for high-concentration protein formulations.

Authors:  Susanne Matheus; Wolfgang Friess; Hanns-Christian Mahler
Journal:  Pharm Res       Date:  2006-05-26       Impact factor: 4.200

7.  Comparison of hydroxylated print additives on antibody microarray performance.

Authors:  Peng Wu; David W Grainger
Journal:  J Proteome Res       Date:  2006-11       Impact factor: 4.466

8.  Inhaled insulin is better absorbed when administered as a dry powder compared to solution in the presence or absence of alkylglycosides.

Authors:  Alamdar Hussain; Quamrul H Majumder; Fakhrul Ahsan
Journal:  Pharm Res       Date:  2006-12-07       Impact factor: 4.200

Review 9.  Effects of glycosylation on the stability of protein pharmaceuticals.

Authors:  Ricardo J Solá; Kai Griebenow
Journal:  J Pharm Sci       Date:  2009-04       Impact factor: 3.534

10.  Role of calcium alginate and mannitol in protecting Bifidobacterium.

Authors:  Dianawati Dianawati; Vijay Mishra; Nagendra P Shah
Journal:  Appl Environ Microbiol       Date:  2012-07-27       Impact factor: 4.792
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