| Literature DB >> 17081047 |
Abstract
Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.Entities:
Mesh:
Substances:
Year: 2006 PMID: 17081047 PMCID: PMC2528199 DOI: 10.1021/pr060217d
Source DB: PubMed Journal: J Proteome Res ISSN: 1535-3893 Impact factor: 4.466