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Literature DB >> 727775

Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent.

Abstract

A sensitive radioimmunoassay utilizing Staphylococcus aureus cells containing protein A as a coprecipitant was developed for the detection and quantitation of staphylococcal enterotoxins A, B, C, D, and E in a variety of foods. The enterotoxins were extracted from the foods by a simple and rapid procedure. The sensitivity of the assay is 1.0 ng or less of enterotoxin per g of food.

Entities: Gene Species

Mesh：

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Year: 1978 PMID： 727775 PMCID： PMC243063 DOI： 10.1128/aem.36.3.421-426.1978

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

1. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors: S W Kessler
Journal: J Immunol Date: 1975-12 Impact factor: 5.422

2. Double-antibody radioimmunoassay for staphylococcal enterotoxin C2.

Authors: H Robern; M Dighton; Y Yano; N Dickie
Journal: Appl Microbiol Date: 1975-10

3. Iodination of staphylococcal enterotoxin B by use of chloramine-T.

Authors: D S Orth
Journal: Appl Environ Microbiol Date: 1977-04 Impact factor: 4.792

4. Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay.

Authors: P E Kauffman; H M Johnson
Journal: Appl Microbiol Date: 1975-06

5. Preparation of labeled staphylococcal enterotoxin A with high specific activity.

Authors: A Niskanen; S Lindroth
Journal: Appl Environ Microbiol Date: 1976-12 Impact factor: 4.792

6. Staphylococcal enterotoxin C: solid-phase radioimmunoassay.

Authors: J A Bukovic; H M Johnson
Journal: Appl Microbiol Date: 1975-10

7. Standardization of the chloramine-T method of protein iodination.

Authors: R Hunter
Journal: Proc Soc Exp Biol Med Date: 1970-03

8. Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.

Authors: W S Collins; A D Johnson; J F Metzger; R W Bennett
Journal: Appl Microbiol Date: 1973-05

9. A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin.

Authors: W S Collins; J F Metzger; A D Johnson
Journal: J Immunol Date: 1972-03 Impact factor: 5.422

10. Statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins A, B, and C.

Authors: D S Orth
Journal: Appl Environ Microbiol Date: 1977-12 Impact factor: 4.792

14 in total

1. Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts.

Authors: J A Orden; J Goyache; J Hernández; A Doménech; G Suárez; E Gómez-Lucía
Journal: Appl Environ Microbiol Date: 1992-12 Impact factor: 4.792

2. Effect of chemical modification of histidine and tyrosine residues in toxic shock syndrome toxin 1 on the serologic and mitogenic activities of the toxin.

Authors: N P Kokan-Moore; M S Bergdoll
Journal: Infect Immun Date: 1989-07 Impact factor: 3.441

3. Incidence of antibodies reactive with toxic shock syndrome toxin 1 in bovine milk.

Authors: N E Thompson; E Gomez-Lucia; M S Bergdoll
Journal: Appl Environ Microbiol Date: 1986-04 Impact factor: 4.792

4. Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems.

Authors: N E Thompson; M Razdan; G Kuntsmann; J M Aschenbach; M L Evenson; M S Bergdoll
Journal: Appl Environ Microbiol Date: 1986-05 Impact factor: 4.792

5. Direct skin test in highly sensitized guinea pigs for rapid and sensitive determination of staphylococcal enterotoxin B.

Authors: P H Scheuber; H Mossmann; G Beck; D K Hammer
Journal: Appl Environ Microbiol Date: 1983-12 Impact factor: 4.792

6. Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments.

Authors: N E Thompson; M J Ketterhagen; M S Bergdoll
Journal: Infect Immun Date: 1984-07 Impact factor: 3.441