Literature DB >> 724495

Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.

V D Axelrod, R M Vartikyan, V A Aivazashvili, R S Beabealashvili.   

Abstract

Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.

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Year:  1978        PMID： 724495      PMCID： PMC342694          DOI： 10.1093/nar/5.10.3549

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host.

Authors:  F W Studier
Journal:  J Mol Biol       Date:  1975-05-15       Impact factor: 5.469

2.  Enzymatic synthesis of deoxyribonucleic acid. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate.

Authors:  H K SCHACHMAN; J ADLER; C M RADDING; I R LEHMAN; A KORNBERG
Journal:  J Biol Chem       Date:  1960-11       Impact factor: 5.157

3.  Separation of oligonucleotides, nucleotides, and nucleosides on columns of polystyrene anion-exchangers with solvent systems containing ethanol.

Authors:  G T Asteriadis; M A Armbruster; P T Gilham
Journal:  Anal Biochem       Date:  1976-01       Impact factor: 3.365

4.  New rapid gel sequencing method for RNA.

Authors:  A Simoncsits; G G Brownlee; R S Brown; J R Rubin; H Guilley
Journal:  Nature       Date:  1977-10-27       Impact factor: 49.962

5.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide.

Authors:  M R Atkinson; M P Deutscher; A Kornberg; A F Russell; J G Moffatt
Journal:  Biochemistry       Date:  1969-12       Impact factor: 3.162

7.  Nucleic acid related compounds. II. A rapid and quantitative preparation of 2'-0- and 3'-0-methyl nucleosides.

Authors:  S R Naik; M J Robins
Journal:  Biochim Biophys Acta       Date:  1971-08-26

8.  Purine nucleosides. XVI. Synthesis of the naturally occurring 2 -O-methylpurine ribonucleosides and related derivatives.

Authors:  T A Khwaja; R K Robins
Journal:  J Am Chem Soc       Date:  1966-08-05       Impact factor: 15.419

9.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  7 in total

1.  Topography of transcription: path of the leading end of nascent RNA through the Escherichia coli transcription complex.

Authors:  M M Hanna; C F Meares
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

2.  Stability of cloned promoter-containing fragments.

Authors:  L P Savochkina; V O Retchinsky; R S Beabealashvilli
Journal:  Mol Gen Genet       Date:  1983

3.  Structure-independent nucleotide sequence analysis.

Authors:  D R Mills; F R Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

4.  Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis.

Authors:  Hameer Ruparel; Lanrong Bi; Zengmin Li; Xiaopeng Bai; Dae Hyun Kim; Nicholas J Turro; Jingyue Ju
Journal:  Proc Natl Acad Sci U S A       Date:  2005-04-13       Impact factor: 11.205

5.  Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.

Authors:  D Rabin; D M Crothers
Journal:  Nucleic Acids Res       Date:  1979-10-10       Impact factor: 16.971

6.  Inhibition of vaccinia virus growth and virus-specific RNA synthesis by 3'-O-methyl adenosine and 3'-O-methyl guanosine.

Authors:  B B Goswami; O K Sharma
Journal:  J Virol       Date:  1983-03       Impact factor: 5.103

7.  Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2'-5' Linkages.

Authors:  Christopher Cozens; Hannes Mutschler; Geoffrey M Nelson; Gillian Houlihan; Alexander I Taylor; Philipp Holliger
Journal:  Angew Chem Int Ed Engl       Date:  2015-11-03       Impact factor: 15.336
  7 in total

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