| Literature DB >> 7192285 |
P H Ellims, M B Van der Weyden.
Abstract
Thymidine kinase (EC 2.7.1.21) has been purified 5600-fold to apparent homogeneity from normal adult human liver. The purification included affinity chromatography on a thymidine column. The subunit molecular weight of the enzyme obtained from this purification was 48,000, and human liver thymidine kinase exhibited association and dissociation. The smallest native molecular form had a molecular weight as determined by gel filtration of 49,000 and the enzyme consistently associated into forms with a molecular weight of 98,000 and 340,000. With isoelectric focusing, the enzyme demonstrated a single form with an isoelectric point of 5.1.Entities:
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Year: 1980 PMID： 7192285
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157