Literature DB >> 6985469

Human thymidine kinase: purification and some properties of the TK1 isoenzyme from placenta.

P H Ellims, T E Gan, L Cosgrove.   

Abstract

Human thymidine kinase TK1 isoenzyme has been purified 1800-fold from placenta to a specific activity of 2.9 nmoles/min/mg of protein. The rapid purification procedure includes affinity chromatography on a thymidine-Sepharose column. At all stages of purification, the enzyme showed irreversible lability. The native molecular weight was determined to be 45000. Human placental TK1 exhibited specificity for ATP and thymidine as substrates, and significant inhibition was found only with thymidine nucleotides. TTP was the most effective inhibitor.

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Year:  1982        PMID： 6985469     DOI： 10.1007/BF00223505

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  19 in total

1.  Induction of thymidine kinases in phytohaemagglutinin-stimulated human lymphocytes.

Authors:  B Munch-Petersen; G Tyrsted
Journal:  Biochim Biophys Acta       Date:  1977-10-04

2.  Thymidine kinase, DNA synthesis and cancer.

Authors:  S Kit
Journal:  Mol Cell Biochem       Date:  1976-06-15       Impact factor: 3.396

3.  Expression of thymidine kinase variants is a function of the replicative state of cells.

Authors:  R Adler; B R McAuslan
Journal:  Cell       Date:  1974-06       Impact factor: 41.582

4.  The presence of "fetal" thymidine kinase in human tumors.

Authors:  M A Stafford; O W Jones
Journal:  Biochim Biophys Acta       Date:  1972-08-25

5.  Properties of thymidine kinase partially purified from human fetal and adult tissue.

Authors:  A T Taylor; M A Stafford; O W Jones
Journal:  J Biol Chem       Date:  1972-03-25       Impact factor: 5.157

Review 6.  Enzymology of cancer cells (first of two parts).

Authors:  G Weber
Journal:  N Engl J Med       Date:  1977-03-03       Impact factor: 91.245

7.  Thymidine kinase isoenzymes in chronic lymphocytic leukaemia.

Authors:  P H Ellims; T E Gan; M B Van der Weyden
Journal:  Br J Haematol       Date:  1981-11       Impact factor: 6.998

8.  Thymidine kinase isoenzymes in human malignant lymphoma.

Authors:  P H Ellims; M B Van der Weyden; G Medley
Journal:  Cancer Res       Date:  1981-02       Impact factor: 12.701

9.  Human liver thymidine kinase. Purification and some properties of the enzyme.

Authors:  P H Ellims; M B Van der Weyden
Journal:  J Biol Chem       Date:  1980-12-10       Impact factor: 5.157

10.  Stabilization of SV40 transformed human fibroblast cytoplasmic thymidine kinase by ATP.

Authors:  P N Porter; D Bull; O W Jones
Journal:  Mol Cell Biochem       Date:  1981-02-26       Impact factor: 3.396
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  3 in total

1.  Human thymidine kinase can functionally replace herpes simplex virus type 1 thymidine kinase for viral replication in mouse sensory ganglia and reactivation from latency upon explant.

Authors:  S H Chen; W J Cook; K L Grove; D M Coen
Journal:  J Virol       Date:  1998-08       Impact factor: 5.103

Review 2.  Thymidine as an anticancer agent, alone or in combination. A biochemical appraisal.

Authors:  P H Ellims
Journal:  Cancer Chemother Pharmacol       Date:  1982-12       Impact factor: 3.333

3.  3'-Amino thymidine affinity matrix for the purification of herpes simplex virus thymidine kinase.

Authors:  P P Tung; J Respass; W C Summers
Journal:  Yale J Biol Med       Date:  1996 Nov-Dec
  3 in total

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