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Literature DB >> 6956858

Partial purification and characterization of the mRNA for human thymidine kinase and hypoxanthine/guanine phosphoribosyltransferase.

P F Lin, M Yamaizumi, P D Murphy, A Egg, F H Ruddle.

Abstract

We used direct microinjection of poly(A)+RNA into individual hypoxanthine/guanine phosphoribosyltransferase-deficient or thymidine kinase-deficient cells and detected the specific in vivo translation products as an assay for human hypoxanthine/guanine phosphoribosyltransferase or thymidine kinase mRNAs. The incorporation of [3H]hypoxanthine or [3H]thymidine into cells in response to injected mRNA was assayed in situ by autoradiography. Methylmercuric hydroxide/agarose gel analysis showed that human hypoxanthine/guanine phosphoribosyltransferase mRNA contains approximately 1,530 nucleotides, which is twice the number required for its protein coding capacity. The mRNA for human cytoplasmic thymidine kinase is estimated to be approximately the same length; thus, the size of the cytosol thymidine kinase subunit can be predicted to be approximately 47,000 daltons, if the full coding capacity of its mRNA is utilized.

Entities: Chemical Disease Gene Species

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Year: 1982 PMID： 6956858 PMCID： PMC346656 DOI： 10.1073/pnas.79.14.4290

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

42 in total

1. Microinjection studies of duck globin messenger RNA translation in human and avian cells.

Authors: D W Stacey; V G Allfrey
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2. Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia.

Authors: L S Lee; Y C Cheng
Journal: J Biol Chem Date: 1976-05-10 Impact factor: 5.157

3. Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.

Authors: W P Summers; M Wagner; W C Summers
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

4. Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels.

Authors: G Spohr; M E Mirault; T Imaizumi; K Scherrer
Journal: Eur J Biochem Date: 1976-02-16

5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors: J M Bailey; N Davidson
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

6. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors: H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

7. Thymidine kinase, DNA synthesis and cancer.

Authors: S Kit
Journal: Mol Cell Biochem Date: 1976-06-15 Impact factor: 3.396

8. Messenger RNA in heat-shocked Drosophila cells.

Authors: A Spradling; M L Pardue; S Penman
Journal: J Mol Biol Date: 1977-02-05 Impact factor: 5.469

9. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.

Authors: M Graessmann; A Graessman
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

10. Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.

Authors: K Gross; E Probst; W Schaffner; M Birnstiel
Journal: Cell Date: 1976-08 Impact factor: 41.582

10 in total

1. Induction of cellular thymidine kinase occurs at the mRNA level.

Authors: P Stuart; M Ito; C Stewart; S E Conrad
Journal: Mol Cell Biol Date: 1985-06 Impact factor: 4.272

2. Partial purification and characterization of the mRNA complementing a temperature-sensitive S-phase cell cycle mutation.

Authors: A Fainsod; M Marcus; P F Lin; F H Ruddle
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

Partial purification and characterization of the mRNA for human thymidine kinase and hypoxanthine/guanine phosphoribosyltransferase.

1. Microinjection studies of duck globin messenger RNA translation in human and avian cells.

2. Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia.

3. Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.

4. Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels.

5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

6. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

7. Thymidine kinase, DNA synthesis and cancer.

8. Messenger RNA in heat-shocked Drosophila cells.

9. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.

10. Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.

1. Induction of cellular thymidine kinase occurs at the mRNA level.

2. Partial purification and characterization of the mRNA complementing a temperature-sensitive S-phase cell cycle mutation.

3. Isolation and preliminary characterization of the Chinese hamster thymidine kinase gene.

4. Human hypoxanthine-guanine phosphoribosyltransferase: studies of the normal and five mutant forms of the enzyme.

5. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.

6. Molecular cloning and cell cycle-specific regulation of a functional human thymidine kinase gene.

7. Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.

8. Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells.

9. Genomic cloning and preliminary characterization of the human thymidine kinase gene.

10. Transient complementation of xeroderma pigmentosum cells by microinjection of poly(A)+ RNA.