Literature DB >> 7179716

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.

P Callebaut, P Debouck, M Pensaert.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected in PED.

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Year:  1982        PMID: 7179716      PMCID: PMC7117127          DOI: 10.1016/0378-1135(82)90009-8

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN: 0378-1135            Impact factor:   3.293


  16 in total

1.  Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus.

Authors:  R L Peters; M J Collins; A J O'Beirne; P A Howton; S L Hourihan; S F Thomas
Journal:  J Clin Microbiol       Date:  1979-10       Impact factor: 5.948

2.  Experimental infection of pigs with Belgian isolates of the porcine rotavirus.

Authors:  P Debouck; M Pensaert
Journal:  Zentralbl Veterinarmed B       Date:  1979-09

3.  Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves.

Authors:  D J Ellens; P W de Leeuw
Journal:  J Clin Microbiol       Date:  1977-11       Impact factor: 5.948

4.  Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies.

Authors:  H B Greenberg; R G Wyatt; J Valdesuso; A R Kalica; W T London; R M Chanock; A Z Kapikian
Journal:  J Med Virol       Date:  1978       Impact factor: 2.327

5.  Simplified radioimmunoassay for detection of human rotavirus in stools.

Authors:  G Cukor; M K Berry; N R Blacklow
Journal:  J Infect Dis       Date:  1978-12       Impact factor: 5.226

6.  Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.

Authors:  D J Ellens; P W de Leeuw; P J Straver; J A van Balken
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402

7.  ELISA for rotavirus.

Authors:  R Yolken; R G Wyatt; A Z Kapikian
Journal:  Lancet       Date:  1977-10-15       Impact factor: 79.321

8.  Microtiter solid-phase radioimmunoassay for hepatitis B antigen.

Authors:  R H Purcell; D C Wong; H J Alter; P V Holland
Journal:  Appl Microbiol       Date:  1973-10

9.  Experimental infection of pigs with a new porcine enteric coronavirus, CV 777.

Authors:  P Debouck; M Pensaert
Journal:  Am J Vet Res       Date:  1980-02       Impact factor: 1.156

10.  Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.

Authors:  C D Brandt; H W Kim; W J Rodriguez; L Thomas; R H Yolken; J O Arrobio; A Z Kapikian; R H Parrott; R M Chanock
Journal:  J Clin Microbiol       Date:  1981-05       Impact factor: 5.948

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  19 in total

1.  Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods.

Authors:  F Guscetti; C Bernasconi; K Tobler; K Van Reeth; A Pospischil; M Ackermann
Journal:  Clin Diagn Lab Immunol       Date:  1998-05

2.  Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus.

Authors:  C F Crouch; T J Raybould; S D Acres
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

3.  Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine epidemic diarrhea virus in pigs.

Authors:  Okjin Kim; Chanhee Chae
Journal:  Can J Vet Res       Date:  2002-04       Impact factor: 1.310

4.  Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA.

Authors:  Priscilla F Gerber; Qiaoling Gong; Yao-Wei Huang; Chong Wang; Derald Holtkamp; Tanja Opriessnig
Journal:  Vet J       Date:  2014-07-24       Impact factor: 2.688

5.  The Carboxy Terminal Region on Spike Protein of Porcine Epidemic Diarrhea Virus (PEDV) Is Important for Evaluating Neutralizing Activity.

Authors:  Ki-Jong Kang; Dong-Hwan Kim; Eui-Ju Hong; Hyun-Jin Shin
Journal:  Pathogens       Date:  2021-05-31

6.  Ultrasensitive Detection of RNA and DNA Viruses Simultaneously Using Duplex UNDP-PCR Assay.

Authors:  Yong Huang; Na Xing; Zengguo Wang; Xiujuan Zhang; Xiaomin Zhao; Qian Du; Lingling Chang; Dewen Tong
Journal:  PLoS One       Date:  2015-11-06       Impact factor: 3.240

7.  Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus.

Authors:  Enrica Sozzi; Andrea Luppi; Davide Lelli; Ana Moreno Martin; Elena Canelli; Emiliana Brocchi; Antonio Lavazza; Paolo Cordioli
Journal:  Res Vet Sci       Date:  2009-06-05       Impact factor: 2.534

8.  Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs.

Authors:  S Houben; P Callebaut; M B Pensaert
Journal:  J Virol Methods       Date:  1995-01       Impact factor: 2.014

9.  Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs.

Authors:  K Tuchiya; T Horimoto; M Azetaka; E Takahashi; S Konishi
Journal:  Vet Microbiol       Date:  1991-01       Impact factor: 3.293

10.  Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus.

Authors:  Seong-Hee Kim; In-Joong Kim; Hyun-Mi Pyo; Dong-Seob Tark; Jae-Young Song; Bang-Hun Hyun
Journal:  J Virol Methods       Date:  2007-08-13       Impact factor: 2.014

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