| Literature DB >> 214679 |
D J Ellens, P W de Leeuw, P J Straver, J A van Balken.
Abstract
Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing.Entities:
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Year: 1978 PMID: 214679 PMCID: PMC7086822 DOI: 10.1007/bf02121145
Source DB: PubMed Journal: Med Microbiol Immunol ISSN: 0300-8584 Impact factor: 3.402