Literature DB >> 214679

Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.

D J Ellens, P W de Leeuw, P J Straver, J A van Balken.   

Abstract

Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing.

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Year:  1978        PMID: 214679      PMCID: PMC7086822          DOI: 10.1007/bf02121145

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN: 0300-8584            Impact factor:   3.402


  18 in total

1.  Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy.

Authors:  J C Bridger; G N Woode
Journal:  Br Vet J       Date:  1975 Sep-Oct

Review 2.  Viral enteritis of calves.

Authors:  G N Woode; J C Bridger
Journal:  Vet Rec       Date:  1975-01-25       Impact factor: 2.695

3.  [Application of enzyme-linked immunosorbent assay (ELISA) to the detection of calf rotavirus and rotavirus antibodies (author's transl)].

Authors:  R Scherrer; S Bernard
Journal:  Ann Microbiol (Paris)       Date:  1977 May-Jun

4.  Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.

Authors:  P J Middleton; M Petric; C M Hewitt; M T Szymanski; J S Tam
Journal:  J Clin Pathol       Date:  1976-03       Impact factor: 3.411

5.  Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.

Authors:  B Tufvesson; T Johnsson
Journal:  Acta Pathol Microbiol Scand B       Date:  1976-08

6.  Solid-phase radioimmunoassay for the detection of rotavirus.

Authors:  P J Middleton; M D Holdaway; M Petric; M T Szymanski; J S Tam
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

7.  A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools.

Authors:  A R Kalica; R H Purcell; M M Sereno; R G Wyatt; H W Kim; R M Chanock; A Z Kapikian
Journal:  J Immunol       Date:  1977-04       Impact factor: 5.422

8.  Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

Authors:  G Wolters; L Kuijpers; J Kacaki; A Schuurs
Journal:  J Clin Pathol       Date:  1976-10       Impact factor: 3.411

9.  Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.

Authors:  M W Peterson; R S Spendlove; R A Smart
Journal:  J Clin Microbiol       Date:  1976-03       Impact factor: 5.948

10.  Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.

Authors:  P C Grauballe; J Genner; A Meyling; A Hornsleth
Journal:  J Gen Virol       Date:  1977-05       Impact factor: 3.891

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  13 in total

1.  Variations and persistency of electropherotypes of bovine rotavirus field isolates. Brief report.

Authors:  N L Fijtman; M E Barrandeguy; E M Cornaglia; A A Schudel
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

2.  Possible sex differences in the susceptibility of calves to rotavirus infection.

Authors:  S A Hasso; R Pandey
Journal:  Can J Vet Res       Date:  1986-04       Impact factor: 1.310

3.  A class capture enzyme immunoassay for immunoglobulin level determinations in bovine sera.

Authors:  K Nielsen; B Rosenbaum; J Stiller
Journal:  Can J Comp Med       Date:  1985-10

4.  Glycoprotein glycans that inhibit adhesion of Escherichia coli mediated by K99 fimbriae: treatment of experimental colibacillosis.

Authors:  M Mouricout; J M Petit; J R Carias; R Julien
Journal:  Infect Immun       Date:  1990-01       Impact factor: 3.441

5.  Neonatal bovine enteritis: detection of rotavirus by counterimmunoelectrophoresis and enzyme-linked immunosorbent assay.

Authors:  L J Ekern; I A Schipper; K J McMahon
Journal:  Can J Comp Med       Date:  1981-04

6.  Detection of porcine rotavirus by EM, ELISA and CIET.

Authors:  J Askaa; B Bloch
Journal:  Acta Vet Scand       Date:  1981       Impact factor: 1.695

7.  Gastroenteritis in infants, associated with a genome type of adenovirus 31 and with combined rotavirus and adenovirus 31 infection.

Authors:  T Adrian; R Wigand; J Richter
Journal:  Eur J Pediatr       Date:  1987-01       Impact factor: 3.183

8.  Rotavirus infections in calves: efficacy of oral vaccination in endemically infected herds.

Authors:  P W de Leeuw; D J Ellens; F P Talmon; G N Zimmer; R Kommerij
Journal:  Res Vet Sci       Date:  1980-09       Impact factor: 2.534

9.  An outbreak of gastroenteritis caused by both rotavirus and Shigella sonnei in a private school in Rio de Janeiro.

Authors:  F Sutmoller; R S Azeredo; M D Lacerda; O M Barth; H G Pereira; E Hoffer; H G Schatzmayr
Journal:  J Hyg (Lond)       Date:  1982-04

10.  Reduction in morbidity due to diarrhea in nursing beef calves by use of an inactivated oil-adjuvanted rotavirus-Escherichia coli vaccine in the dam.

Authors:  E M Cornaglia; F M Fernández; M Gottschalk; M E Barrandeguy; A Luchelli; M I Pasini; L J Saif; J R Parraud; A Romat; A A Schudel
Journal:  Vet Microbiol       Date:  1992-02       Impact factor: 3.293

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