Literature DB >> 6325490

Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus.

C F Crouch, T J Raybould, S D Acres.   

Abstract

Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, highly specific, and reproducible assay for the detection of bovine enteric coronavirus was developed, using a mixture of two of these monoclonal antibodies reactive with antigenic components either external or internal to the virion. These monoclonal antibodies were bound indirectly to 96-well plates via rabbit anti-mouse immunoglobulin. After sample application and incubation, virus was detected by using rabbit anti-coronavirus peroxidase conjugate followed by enzyme substrate and chromagen. Fecal samples from a single herd of cows were screened for the presence of coronavirus by this assay. Five percent of clinically normal cows were found to be shedding coronavirus.

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Year:  1984        PMID： 6325490      PMCID： PMC271071          DOI： 10.1128/jcm.19.3.388-393.1984

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

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  21 in total

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10.  Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.

Authors:  Xiao-Yan Che; Li-Wen Qiu; Yu-Xian Pan; Kun Wen; Wei Hao; Li-Ya Zhang; Ya-Di Wang; Zhi-Yong Liao; Xu Hua; Vincent C C Cheng; Kwok-Yung Yuen
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948
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