Literature DB >> 7730433

Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs.

S Houben1, P Callebaut, M B Pensaert.   

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to the porcine reproductive and respiratory syndrome virus (PRRSV) in pig sera was developed and compared with the immunoperoxidase monolayer assay (IPMA), the most widely used serological diagnostic test in Europe. The blocking ELISA was specific and more sensitive than the IPMA when applied to field sera and to sera which were collected early after an experimental infection with PRRSV. Problems with high background activity as observed in IPMA or indirect ELISA were not encountered.

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Year:  1995        PMID： 7730433      PMCID： PMC7119743          DOI： 10.1016/0166-0934(94)00135-4

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  3 in total

1.  An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus.

Authors:  E Albina; Y Leforban; T Baron; J P Plana Duran; P Vannier
Journal:  Ann Rech Vet       Date:  1992

2.  Mystery swine disease in The Netherlands: the isolation of Lelystad virus.

Authors:  G Wensvoort; C Terpstra; J M Pol; E A ter Laak; M Bloemraad; E P de Kluyver; C Kragten; L van Buiten; A den Besten; F Wagenaar
Journal:  Vet Q       Date:  1991-07       Impact factor: 3.320

3.  Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.

Authors:  P Callebaut; P Debouck; M Pensaert
Journal:  Vet Microbiol       Date:  1982-09       Impact factor: 3.293
  3 in total
  8 in total

1.  Performance of ELISA antigens prepared from 8 isolates of porcine reproductive and respiratory syndrome virus with homologous and heterologous antisera.

Authors:  H J Cho; S C Entz; R Magar; H S Joo
Journal:  Can J Vet Res       Date:  1997-10       Impact factor: 1.310

2.  Evaluation of newly developed immunoperoxidase monolayer assays for detection of antibodies against bovine herpesvirus 4.

Authors:  G J Wellenberg; E M van Rooij; J Maissan; J T Van Oirschot
Journal:  Clin Diagn Lab Immunol       Date:  1999-07

3.  Comparative study of serological methods for the detection of antibodies to porcine reproductive and respiratory syndrome virus.

Authors:  H J Cho; B McNab; C Dubuc; L Jordan; A Afshar; R Magar; S Prins; K Eernisse
Journal:  Can J Vet Res       Date:  1997-07       Impact factor: 1.310

4.  Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus.

Authors:  Neal H Ferrin; Ying Fang; Craig R Johnson; Michael P Murtaugh; Dale D Polson; Montserrat Torremorell; Marie L Gramer; Eric A Nelson
Journal:  Clin Diagn Lab Immunol       Date:  2004-05

5.  Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus.

Authors:  Jie Li; Gang Wang; Di Yang; Bao Zhao; Yongpan Zhao; Yonggang Liu; Xuehui Cai; Yuchen Nan; En-Min Zhou; Chunyan Wu
Journal:  BMC Biotechnol       Date:  2018-11-16       Impact factor: 2.563

6.  Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on a request from the Commission related to the probability of transmission of Porcine Reproductive and Respiratory Syndrome virus (PRRSv) to naive pigs via fresh meat.

Authors: 
Journal:  EFSA J       Date:  2005-08-12

Review 7.  Immunity to PRRSV: double-edged sword.

Authors:  T W Molitor; E M Bautista; C S Choi
Journal:  Vet Microbiol       Date:  1997-04       Impact factor: 3.293

Review 8.  Porcine reproductive and respiratory syndrome (PRRS): a review, with emphasis on pathological, virological and diagnostic aspects.

Authors:  S H Done; D J Paton; M E White
Journal:  Br Vet J       Date:  1996-03
  8 in total

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