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Literature DB >> 708033

Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation.

Abstract

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.

Entities: Chemical Species

Mesh：

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Year: 1978 PMID： 708033 PMCID： PMC243104 DOI： 10.1128/aem.36.4.625-626.1978

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

6 in total

1. Extraction, purification, and properties of Bacillus sotto toxin.

Authors: T A ANGUS
Journal: Can J Microbiol Date: 1956-06 Impact factor: 2.419

2. Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

Authors: E S Sharpe; K W Nickerson; L A Bulla; J N Aronson
Journal: Appl Microbiol Date: 1975-12

3. Dipicolinic acid-less mutants of Bacillus cereus.

Authors: J Wise; A Swanson; H O Halvorson
Journal: J Bacteriol Date: 1967-12 Impact factor: 3.490

4. Density gradient centrifugation for the separation of sporulating forms of bacteria.

Authors: H Tamir; C Gilvarg
Journal: J Biol Chem Date: 1966-03-10 Impact factor: 5.157

5. Immunological homology between crystal and spore protein of Bacillus thuringiensis.

Authors: F P Delafield; H J Somerville; S C Rittenberg
Journal: J Bacteriol Date: 1968-09 Impact factor: 3.490

6. Biphasic system for separation of spores and crystals of Bacillus thuringiensis.

Authors: N S Goodman; R J Gottfried; M H Rogoff
Journal: J Bacteriol Date: 1967-08 Impact factor: 3.490

6 in total

20 in total

1. Formation of crystals of the insecticidal proteins of Bacillus thuringiensis subsp. aizawai IPL7 in Escherichia coli.

Authors: K Oeda; K Inouye; Y Ibuchi; K Oshie; M Shimizu; K Nakamura; R Nishioka; Y Takada; H Ohkawa
Journal: J Bacteriol Date: 1989-06 Impact factor: 3.490

2. The glycoprotein toxin of Bacillus thuringiensis subsp. israelensis indicates a lectinlike receptor in the larval mosquito gut.

Authors: G Muthukumar; K W Nickerson
Journal: Appl Environ Microbiol Date: 1987-11 Impact factor: 4.792

3. Nonenzymatic Glycosylation of Lepidopteran-Active Bacillus thuringiensis Protein Crystals.

Authors: M Bhattacharya; B A Plantz; J D Swanson-Kobler; K W Nickerson
Journal: Appl Environ Microbiol Date: 1993-08 Impact factor: 4.792

4. Separation of Protein Crystals from Spores of Bacillus thuringiensis by Ludox Gradient Centrifugation.

Authors: Y S Zhu; A Brookes; K Carlson; P Filner
Journal: Appl Environ Microbiol Date: 1989-05 Impact factor: 4.792

5. Comparison of Disulfide Contents and Solubility at Alkaline pH of Insecticidal and Noninsecticidal Bacillus thuringiensis Protein Crystals.

Authors: C Du; P A Martin; K W Nickerson
Journal: Appl Environ Microbiol Date: 1994-10 Impact factor: 4.792

6. Dissolution and Degradation of Bacillus thuringiensis delta-Endotoxin by Gut Juice Protease of the Silkworm Bombyx mori.

Authors: A Tojo; K Aizawa
Journal: Appl Environ Microbiol Date: 1983-02 Impact factor: 4.792

7. Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide.

Authors: K W Nickerson
Journal: Appl Environ Microbiol Date: 1982-05 Impact factor: 4.792

8. Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. "kurstaki" and "israelensis".

Authors: L Thorne; F Garduno; T Thompson; D Decker; M Zounes; M Wild; A M Walfield; T J Pollock
Journal: J Bacteriol Date: 1986-06 Impact factor: 3.490

9. Bacillus thuringiensis HD-73 Spores Have Surface-Localized Cry1Ac Toxin: Physiological and Pathogenic Consequences.

Authors: C Du; K W Nickerson
Journal: Appl Environ Microbiol Date: 1996-10 Impact factor: 4.792

10. Toxicity of Bacillus thuringiensis var. israelensis crystals to Aedes aegypti larvae: carbonate reversal.

Authors: D J Schnell; K W Nickerson
Journal: Appl Environ Microbiol Date: 1983-05 Impact factor: 4.792