Literature DB >> 16347918

Separation of Protein Crystals from Spores of Bacillus thuringiensis by Ludox Gradient Centrifugation.

Y S Zhu1, A Brookes, K Carlson, P Filner.   

Abstract

A method is described for the purification of Bacillus thuringiensis protein crystals by Ludox gradient centrifugation. This method is simple, inexpensive, fast, and efficient compared with other techniques. It has been successfully used to purify and characterize the protein crystals from several B. thuringiensis strains.

Entities:  

Year:  1989        PMID： 16347918      PMCID： PMC184290          DOI： 10.1128/aem.55.5.1279-1281.1989

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

Authors:  E S Sharpe; K W Nickerson; L A Bulla; J N Aronson
Journal:  Appl Microbiol       Date:  1975-12

2.  Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation.

Authors:  B J Ang; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1978-10       Impact factor: 4.792

3.  Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai IC1 toxic to both lepidoptera and diptera.

Authors:  M Z Haider; E S Ward; D J Ellar
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Biphasic system for separation of spores and crystals of Bacillus thuringiensis.

Authors:  N S Goodman; R J Gottfried; M H Rogoff
Journal:  J Bacteriol       Date:  1967-08       Impact factor: 3.490
  4 in total
  4 in total

1.  Dot Blot Enzyme-Linked Immunosorbent Assay for Monitoring the Fate of Insecticidal Toxins from Bacillus thuringiensis in Soil.

Authors:  H Tapp; G Stotzky
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

2.  Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores.

Authors:  Milada Ikanovic; Walter E Rudzinski; John G Bruno; Amity Allman; Maria P Carrillo; Sulatha Dwarakanath; Suneetha Bhahdigadi; Poornima Rao; Johnathan L Kiel; Carrie J Andrews
Journal:  J Fluoresc       Date:  2007-01-31       Impact factor: 2.217

3.  Insecticidal activity of Bacillus thuringiensis strains isolated from soil and water.

Authors:  Edyta Konecka; Jakub Baranek; Anita Hrycak; Adam Kaznowski
Journal:  ScientificWorldJournal       Date:  2012-05-01

4.  Human cell exposure assays of Bacillus thuringiensis commercial insecticides: production of Bacillus cereus-like cytolytic effects from outgrowth of spores.

Authors:  A F Tayabali; V L Seligy
Journal:  Environ Health Perspect       Date:  2000-10       Impact factor: 9.031
  4 in total

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