Literature DB >> 16346018

Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide.

K W Nickerson1.   

Abstract

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-beta-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.

Entities:  

Year:  1982        PMID： 16346018      PMCID： PMC244208          DOI： 10.1128/aem.43.5.1208-1209.1982

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  STRUCTURE OF POLY-BETA-HYDROXYBUTYRIC ACID GRANULES.

Authors:  D G LUNDGREN; R M PFISTER; J M MERRICK
Journal:  J Gen Microbiol       Date:  1964-03

2.  Assay of poly-beta-hydroxybutyric acid.

Authors:  J H LAW; R A SLEPECKY
Journal:  J Bacteriol       Date:  1961-07       Impact factor: 3.490

3.  The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species.

Authors:  D H WILLIAMSON; J F WILKINSON
Journal:  J Gen Microbiol       Date:  1958-08

Review 4.  The role and regulation of energy reserve polymers in micro-organisms.

Authors:  E A Dawes; P J Senior
Journal:  Adv Microb Physiol       Date:  1973       Impact factor: 3.517

5.  Physiology of sporeforming bacteria associated with insects: radiorespirometric survey of carbohydrate metabolism in the 12 serotypes of Bacillus thuringiensis.

Authors:  K W Nickerson; G St Julian; L A Bulla
Journal:  Appl Microbiol       Date:  1974-07

6.  Metabolism of poly-beta-hydroxybutyrate. I. Purification, composition, and properties of native poly-beta-hydroxybutyrate granules from Bacillus megaterium.

Authors:  R Griebel; Z Smith; J M Merrick
Journal:  Biochemistry       Date:  1968-10       Impact factor: 3.162

7.  Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation.

Authors:  B J Ang; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1978-10       Impact factor: 4.792

8.  The aerobic pseudomonads: a taxonomic study.

Authors:  R Y Stanier; N J Palleroni; M Doudoroff
Journal:  J Gen Microbiol       Date:  1966-05

9.  Metabolism of poly-beta-hydroxybutyrate and acetoin in Bacillus cereus.

Authors:  L A Kominek; H O Halvorson
Journal:  J Bacteriol       Date:  1965-11       Impact factor: 3.490
  9 in total
  6 in total

1.  Analysis of Poly-beta-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection.

Authors:  D B Karr; J K Waters; D W Emerich
Journal:  Appl Environ Microbiol       Date:  1983-12       Impact factor: 4.792

2.  Determination of Poly-beta-Hydroxybutyrate and Poly-beta-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography.

Authors:  Y Comeau; K J Hall; W K Oldham
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

Review 3.  Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.

Authors:  A J Anderson; E A Dawes
Journal:  Microbiol Rev       Date:  1990-12

4.  Mathematical model for determining the effects of intracytoplasmic inclusions on volume and density of microorganisms.

Authors:  J Mas; C Pedrós-Alió; R Guerrero
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

5.  Analysis of mosquito larvicidal potential exhibited by vegetative cells of Bacillus thuringiensis subsp. israelensis.

Authors:  C J Walther; G A Couche; M A Pfannenstiel; S E Egan; L A Bivin; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

6.  An investigation of bacillus thuringiensis in rectal-collected fecal samples of cows.

Authors:  David R Ammons; Antonio Reyna; Jose Cristobal Granados; Michael S Samlal; Joanne N Rampersad
Journal:  Curr Microbiol       Date:  2009-08-18       Impact factor: 2.188
  6 in total

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