Literature DB >> 7003550

Sequence-specific interactions of the tight-binding I12-X86 lac repressor with non-operator DNA.

A Schmitz, D J Galas.   

Abstract

The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to the inducer IPTG. One strong binding site in the I gene DNA was found to be one of two expected on the basis of their homology with the lac operator. The binding of I12-X86 repressor to this site was visualized using the footprinting technique, and found to be consistent with an operator-like binding configuration. The protection pattern extends into an adjacent sequence suggesting that two repressor tetramers are bound in tandem.

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Year:  1980        PMID： 7003550      PMCID： PMC327285          DOI： 10.1093/nar/8.3.487

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

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5.  Molecular parameters characterizing the interaction of Escherichia coli lac repressor with non-operator DNA and inducer.

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Journal:  Biochemistry       Date:  1977-11-01       Impact factor: 3.162

6.  The nature of lactose operator constitive mutations.

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7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  A hypothesis on a specific sequence-dependent conformation of DNA and its relation to the binding of the lac-repressor protein.

Authors:  A Klug; A Jack; M A Viswamitra; O Kennard; Z Shakked; T A Steitz
Journal:  J Mol Biol       Date:  1979-07-15       Impact factor: 5.469

9.  Tight-binding repressors of the lactose operon.

Authors:  J L Betz; J R Sadler
Journal:  J Mol Biol       Date:  1976-08-05       Impact factor: 5.469

10.  Interaction of int protein with specific sites on lambda att DNA.

Authors:  W Ross; A Landy; Y Kikuchi; H Nash
Journal:  Cell       Date:  1979-10       Impact factor: 41.582
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  10 in total

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6.  Avian retrovirus pp32 DNA-binding protein. I. Recognition of specific sequences on retrovirus DNA terminal repeats.

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Journal:  J Virol       Date:  1982-10       Impact factor: 5.103

7.  Cyclic AMP receptor proteins interacts with lactose operator DNA.

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Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

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