Literature DB >> 6896089

Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer.

P A Krieg, A J Robins, M J Gait, R C Titmas, J R Wells.   

Abstract

We describe the use of a synthetic primer to select a cDNA recombinant clone containing H5 coding sequences. The strategy used was as follows: 1. Prepare oligo(dT) cellulose-bound mRNA from chicken reticulocytes and select 11S-18S material from sucrose gradients. 2. Use this RNA fraction both to prepare a cDNA library and as a template for H5-specific cDNA synthesis using a synthetic primer. 3. Screen out most globin cDNA recombinants with oligo(dT)-primed globin cDNA. 4. Search for H5 recombinants using H5 specific cDNA and verify the identity by DNA sequencing. Our screening suggests an H5 mRNA abundance of about two parts per thousand in chicken reticulocyte poly(A)-containing RNA. The isolation of an H5 cDNA recombinant clone is an initial step in the study of H5 genes and their relationship to H1 and core histone genes.

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Year:  1982        PMID： 6896089      PMCID： PMC320545          DOI： 10.1093/nar/10.5.1495

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Histone changes during chromatin remodeling in embryogenesis.

Authors:  K M Newrock; C R Alfageme; R V Nardi; L H Cohen
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

2.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

3.  Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

Authors:  A C Chang; J H Nunberg; R J Kaufman; H A Erlich; R T Schimke; S N Cohen
Journal:  Nature       Date:  1978-10-19       Impact factor: 49.962

4.  Changes in histone acetyl content and in nuclear non-histone protein composition of avian erythroid cells at different stages of maturation.

Authors:  A Ruiz-Carrillo; L J Wangh; V C Littau; V G Allfrey
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

5.  Synthesis and turnover of DNA-bound histone during maturation of avian red blood cells.

Authors:  R Appels; J R Wells
Journal:  J Mol Biol       Date:  1972-10-14       Impact factor: 5.469

6.  A study of the quantitative variation of histones, and their relationship to RNA synthesis, during erythropoiesis in the adult chicken.

Authors:  M A Billett; J Hindley
Journal:  Eur J Biochem       Date:  1972-08-04

7.  Molecular cloning and sequence analysis of adult chicken betal globin cDNA.

Authors:  R I Richards; J Shine; A Ullrich; J R Wells; H M Goodman
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

8.  Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors:  R Roychoudhury; E Jay; R Wu
Journal:  Nucleic Acids Res       Date:  1976-04       Impact factor: 16.971

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Characterization of DNA-bound histone in the cells of the avian erythropoietic series.

Authors:  R Appels; J R Wells; A F Williams
Journal:  J Cell Sci       Date:  1972-01       Impact factor: 5.285
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  8 in total

1.  The organisation and expression of histone genes from Xenopus borealis.

Authors:  P C Turner; E B Bagenal; M T Vlad; H R Woodland
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

2.  Opposite replication polarities of transcribed and nontranscribed histone H5 genes.

Authors:  J P Trempe; Y I Lindstrom; M Leffak
Journal:  Mol Cell Biol       Date:  1988-04       Impact factor: 4.272

3.  Structure and in vitro transcription of a human H4 histone gene.

Authors:  F Sierra; G Stein; J Stein
Journal:  Nucleic Acids Res       Date:  1983-10-25       Impact factor: 16.971

4.  The chicken H5 gene is unlinked to core and H1 histone genes.

Authors:  P A Krieg; A J Robins; R D'Andrea; J R Wells
Journal:  Nucleic Acids Res       Date:  1983-02-11       Impact factor: 16.971

5.  H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.

Authors:  R P Harvey; J A Whiting; L S Coles; P A Krieg; J R Wells
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

6.  Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences.

Authors:  I Marta Evans; J A Gatehouse; G W Lycett; D Boulter
Journal:  Plant Mol Biol       Date:  1984-03       Impact factor: 4.076

7.  Chicken histone H5 mRNA: the polyadenylated RNA lacks the conserved histone 3' terminator sequence.

Authors:  P A Krieg; A J Robins; A Colman; J R Wells
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

8.  DNA methylation affects nuclear organization, histone modifications, and linker histone binding but not chromatin compaction.

Authors:  Nick Gilbert; Inga Thomson; Shelagh Boyle; James Allan; Bernard Ramsahoye; Wendy A Bickmore
Journal:  J Cell Biol       Date:  2007-05-07       Impact factor: 10.539
  8 in total

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