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Literature DB >> 514809

Molecular cloning and sequence analysis of adult chicken betal globin cDNA.

R I Richards, J Shine, A Ullrich, J R Wells, H M Goodman.

Abstract

The molecular cloning and nucleotide sequence analysis of adult chicken beta globin mRNA is reported. DNA sequences derived from in vitro transcrption of globin mRNA were purified and amplified as recombinant DNA using the plasmid pBR322. Sequence analysis of several clones coding for beta globin strongly suggests that transcription errors may be generated near the 5' end of transcripts in vitro by reverse transcription. The complete sequence of the longest beta globin insert containing 51 bases of the 5' untranslated region as well as the complete coding and 3' untranslated regions has been determined.

Entities: Gene Species

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Year: 1979 PMID： 514809 PMCID： PMC342292 DOI： 10.1093/nar/7.5.1137

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns.

Authors: J van den Berg; A van Ooyen; N Mantei; A Schamböck; G Grosveld; R A Flavell; C Weissmann
Journal: Nature Date: 1978-11-02 Impact factor: 49.962

Molecular cloning and sequence analysis of adult chicken betal globin cDNA.

1. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns.

2. Molecular evolution of human and rabbit beta-globin mRNAs.

Review 3. Recent developments in the molecular genetics of human hemoglobin.

4. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

5. Identification of a new chicken alpha-globin structural gene by complementary DNA cloning.

6. AUG is the only recognisable signal sequence in the 5' non-coding regions of eukaryotic mRNA.

7. Short half-life precursor of globin messenger RNA from chicken erythroblasts.

8. Control of globin gene expression by steroid hormones in differentiating Friend leukemia cells.

9. Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA.

10. Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.

1. Optimal sequence alignments.

2. Rates of nuclear DNA evolution in pheasant-like birds: evidence from restriction maps.

3. Molecular cloning of human synovial cell collagenase and selection of a single gene from genomic DNA.

4. A measure of the similarity of sets of sequences not requiring sequence alignment.

5. Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.

6. Inter-RNA homology and possible roles of small RNAs.

7. Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.

8. Complete nucleotide sequence of the haemagglutinin gene from a human influenza virus of the Hong Kong subtype.

9. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

10. Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA.