Literature DB >> 6743291

Serum albumin enhances the uptake of [3H]cholesterol from phosphatidylcholine vesicles by cultured human fibroblasts.

J P Slotte, S Björkerud.   

Abstract

Cultured human lung fibroblasts, incubated with cholesterol/phosphatidylcholine vesicles (cholesterol: phosphatidylcholine molar ratio 1:1) incorporated vesicle [3H]-cholesterol linearly for at least 48 h by an exchange process without gaining sterol mass. The incorporation of [3H]cholesterol by the cells was markedly enhanced in the presence of purified bovine serum albumin. A fraction of the incorporated vesicle [3H]cholesterol was esterified by the cells.

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Year:  1984        PMID: 6743291      PMCID: PMC1153667          DOI: 10.1042/bj2200605

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  12 in total

1.  Preparation of delipidized serum protein for use in cell culture systems.

Authors:  G H Rothblat; L Y Arbogast; L Ouellette; B V Howard
Journal:  In Vitro       Date:  1976-08

2.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors:  M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

3.  The effect of serum protein fractions on liposome-cell interactions in cultured cells and the perfused rat liver.

Authors:  D A Tyrrell; V J Richardson; B E Ryman
Journal:  Biochim Biophys Acta       Date:  1977-04-27

4.  Interaction of liposomes with cultured cells: effect of serum.

Authors:  E Mayhew; C Gotfredsen; Y J Schneider; A Trouet
Journal:  Biochem Pharmacol       Date:  1980-03-15       Impact factor: 5.858

5.  Mechanism of cholesterol efflux from cells. Effects of acceptor structure and concentration.

Authors:  G H Rothblat; M C Phillips
Journal:  J Biol Chem       Date:  1982-05-10       Impact factor: 5.157

6.  Symmetrical distribution and rapid transbilayer movement of cholesterol in Mycoplasma gallisepticum membranes.

Authors:  S Rottem; D Shinar; R Bittman
Journal:  Biochim Biophys Acta       Date:  1981-12-21

7.  Mechanism of cholesterol and phosphatidylcholine exchange or transfer between unilamellar vesicles.

Authors:  L R McLean; M C Phillips
Journal:  Biochemistry       Date:  1981-05-12       Impact factor: 3.162

8.  Cholesterol movement between human skin fibroblasts and phosphatidylcholine vesicles.

Authors:  M J Poznansky; S Czekanski
Journal:  Biochim Biophys Acta       Date:  1982-02-23

9.  Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

Authors:  W Gamble; M Vaughan; H S Kruth; J Avigan
Journal:  J Lipid Res       Date:  1978-11       Impact factor: 5.922

10.  Plasma membrane lipids of human diploid fibroblasts from normal individuals and patients with cystic fibrosis.

Authors:  J R Riordan; N Alon; M Buchwald
Journal:  Biochim Biophys Acta       Date:  1979-07-27
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  4 in total

1.  Uptake and esterification of exogenous cholesterol by low-density-lipoprotein-receptor-negative human fibroblasts in culture.

Authors:  J P Slotte; S Ekman; S Björkerud
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

2.  Movement of plasma-membrane sterols to the endoplasmic reticulum in cultured cells.

Authors:  J P Slotte; E L Bierman
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

3.  Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp.

Authors:  R G Cluss; N L Somerson
Journal:  Appl Environ Microbiol       Date:  1986-02       Impact factor: 4.792

4.  Interaction of rabbit lipoproteins and red blood cells with liposomes of egg yolk phospholipids.

Authors:  A J Mendez; J L He; H S Huang; S R Wen; S L Hsia
Journal:  Lipids       Date:  1988-10       Impact factor: 1.880

  4 in total

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