Literature DB >> 731127

Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

W Gamble, M Vaughan, H S Kruth, J Avigan.   

Abstract

Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with p-hydroxyphenylacetic acid to yield a stable fluorescent product. Cholesterol ester hydrolase is included for determination of total cholesterol. When samples of sonified cell suspensions are used directly, the extraction of lipids is avoided, permitting one person to carry out analyses of 30 or more subcultures in one day.

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Year:  1978        PMID： 731127

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  121 in total

1.  Rafts promote assembly and atypical targeting of a nonenveloped virus, rotavirus, in Caco-2 cells.

Authors:  Catherine Sapin; Odile Colard; Olivier Delmas; Cedric Tessier; Michelyne Breton; Vincent Enouf; Serge Chwetzoff; Jocelyne Ouanich; Jean Cohen; Claude Wolf; Germain Trugnan
Journal:  J Virol       Date:  2002-05       Impact factor: 5.103

2.  Targeting of Shiga toxin B-subunit to retrograde transport route in association with detergent-resistant membranes.

Authors:  T Falguières; F Mallard; C Baron; D Hanau; C Lingwood; B Goud; J Salamero; L Johannes
Journal:  Mol Biol Cell       Date:  2001-08       Impact factor: 4.138

3.  Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role of distinct lipid microdomains.

Authors:  Tounsia Aït Slimane; Germain Trugnan; Sven C D Van IJzendoorn; Dick Hoekstra
Journal:  Mol Biol Cell       Date:  2003-02       Impact factor: 4.138

4.  G(M2)-ganglioside metabolism in situ in mucolipidosis IV fibroblasts.

Authors:  S Raghavan; E Leshinsky; E H Kolodny
Journal:  Neurochem Res       Date:  1999-04       Impact factor: 3.996

5.  A simple and sensitive enzymatic method for cholesterol quantification in macrophages and foam cells.

Authors:  Peggy Robinet; Zeneng Wang; Stanley L Hazen; Jonathan D Smith
Journal:  J Lipid Res       Date:  2010-08-04       Impact factor: 5.922

6.  Activity of BK(Ca) channel is modulated by membrane cholesterol content and association with Na+/K+-ATPase in human melanoma IGR39 cells.

Authors:  Nobuyoshi Tajima; Yutaka Itokazu; Esa R Korpi; Pentti Somerharju; Reijo Käkelä
Journal:  J Biol Chem       Date:  2010-12-06       Impact factor: 5.157

7.  Heterogeneity of rat liver parenchyma in cholesterol 7 alpha-hydroxylase and bile acid synthesis.

Authors:  B Ugele; H J Kempen; J M Kempen; R Gebhardt; P Meijer; H J Burger; H M Princen
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

8.  Dexamethasone regulates bile acid synthesis in monolayer cultures of rat hepatocytes by induction of cholesterol 7 alpha-hydroxylase.

Authors:  H M Princen; P Meijer; B Hofstee
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

9.  Separate transport systems for biliary secretion of sulfated and unsulfated bile acids in the rat.

Authors:  F Kuipers; M Enserink; R Havinga; A B van der Steen; M J Hardonk; J Fevery; R J Vonk
Journal:  J Clin Invest       Date:  1988-05       Impact factor: 14.808

10.  Ketoconazole blocks bile acid synthesis in hepatocyte monolayer cultures and in vivo in rat by inhibiting cholesterol 7 alpha-hydroxylase.

Authors:  H M Princen; C M Huijsmans; F Kuipers; R J Vonk; H J Kempen
Journal:  J Clin Invest       Date:  1986-10       Impact factor: 14.808
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