Literature DB >> 992668

Preparation of delipidized serum protein for use in cell culture systems.

G H Rothblat, L Y Arbogast, L Ouellette, B V Howard.   

Abstract

A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.

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Year:  1976        PMID： 992668     DOI： 10.1007/bf02797438

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  17 in total

1.  A simple method for the isolation and purification of total lipides from animal tissues.

Authors:  J FOLCH; M LEES; G H SLOANE STANLEY
Journal:  J Biol Chem       Date:  1957-05       Impact factor: 5.157

2.  Separation and characterization of the protein moiety of human alpha1-lipoprotein.

Authors:  A SCANU; L A LEWIS; F M BUMPUS
Journal:  Arch Biochem Biophys       Date:  1958-04       Impact factor: 4.013

3.  Regulation of cellular sterol flux and synthesis by human serum lipoproteins.

Authors:  S R Bates; G H Rothblat
Journal:  Biochim Biophys Acta       Date:  1974-07-26

4.  Regulation of cholesterol synthesis in normal and malignant tissue.

Authors:  M S Brown; J L Goldstein; M D Siperstein
Journal:  Fed Proc       Date:  1973-12

5.  Cholesteryl ester metabolism in tissue culture cells. I. Accumulation in Fu5AH rat hepatoma cells.

Authors:  G H Rothblat
Journal:  Lipids       Date:  1974-08       Impact factor: 1.880

6.  Cholesterol biosynthesis in WI-38 and WI-38VA13A tissue culture cells.

Authors:  G H Rothblat; R Boyd; C Deal
Journal:  Exp Cell Res       Date:  1971-08       Impact factor: 3.905

7.  Control of growth of axemic cultures of mammalian cells by serum lipids.

Authors:  G L Tritsch; J A Bell; G Grahl-Nielsen
Journal:  Nature       Date:  1968-07-20       Impact factor: 49.962

8.  Quantitation of serum lipids by a simple TLC-charring method.

Authors:  D Kritchevsky; L M Davidson; H K Kim
Journal:  Clin Chim Acta       Date:  1973-06-14       Impact factor: 3.786

9.  Control of sterol synthesis and of hydroxymethylglutaryl CoA reductase in skin fibroblasts grown from patients with homozygous type II hyperlipoproteinemia.

Authors:  J Avigan; S J Bhathena; M E Schreiner
Journal:  J Lipid Res       Date:  1975-03       Impact factor: 5.922

10.  Lipids of cultured hepatoma cells. I. Effect of serum lipid levels on cell and media lipids.

Authors:  R Wood
Journal:  Lipids       Date:  1973-12       Impact factor: 1.880
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  41 in total

1.  Relative insensitivity to glucagon of sterol synthesis in cultured rat aortic smooth muscle cells. Effect of dibutyryl cyclic AMP.

Authors:  R W Stout
Journal:  Diabetologia       Date:  1978-10       Impact factor: 10.122

2.  Normal human epidermal keratinocytes express in vitro specific molecular forms of (pro)filaggrin recognized by rheumatoid arthritis-associated antifilaggrin autoantibodies.

Authors:  E Girbal-Neuhauser; M Montézin; F Croute; M Sebbag; M Simon; J J Durieux; G Serre
Journal:  Mol Med       Date:  1997-02       Impact factor: 6.354

3.  Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells.

Authors:  Akiko Obinata; Yoshihiro Akimoto; Hiroshi Hirano; Hiroyoshi Endo
Journal:  Rouxs Arch Dev Biol       Date:  1991-11

4.  Differentiation of normal and tumoral human keratinocytes cultured on dermis: reconstruction of either normal or tumoral architecture.

Authors:  M Regnier; C Desbas; C Bailly; M Darmon
Journal:  In Vitro Cell Dev Biol       Date:  1988-07

5.  Overproduction of a Mr 92,000 protomer of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in compactin-resistant C100 cells.

Authors:  E C Hardeman; H S Jenke; R D Simoni
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

6.  The perinuclear factor, a rheumatoid arthritis-specific autoantigen, is not present in keratohyalin granules of cultured buccal mucosa cells.

Authors:  R M Hoet; R A Voorsmit; W J Van Venrooij
Journal:  Clin Exp Immunol       Date:  1991-04       Impact factor: 4.330

7.  Retention of human skin fibroblast fatty acid modifications during maintenance culture.

Authors:  A A Spector; G M Denning; L L Stoll
Journal:  In Vitro       Date:  1980-11

8.  Selectivity in incorporation, utilization and retention of oleic and linoleic acids by human skin fibroblasts.

Authors:  M D Rosenthal
Journal:  Lipids       Date:  1980-10       Impact factor: 1.880

9.  A serum protein inhibitor of acid lipase and its possible role in lipid accumulation in cultured fibroblasts.

Authors:  E Gorin; H Gonen; S Dickbuch
Journal:  Biochem J       Date:  1982-04-15       Impact factor: 3.857

10.  Origin of fatty acids of cholesteryl ester accumulated by Fu5AH cells in culture.

Authors:  J M Rosen; G H Rothblat
Journal:  Lipids       Date:  1977-02       Impact factor: 1.880
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