Literature DB >> 6487273

Uptake and esterification of exogenous cholesterol by low-density-lipoprotein-receptor-negative human fibroblasts in culture.

J P Slotte, S Ekman, S Björkerud.   

Abstract

The incorporation and metabolism of both vesicle- and LDL (low-density lipoprotein)- derived [3H]cholesterol by LDL-receptor-negative fibroblasts were studied. Independent of the cholesterol source, free [3H]cholesterol was readily incorporated into the cells and was available for esterification. 7-Oxocholesterol stimulated both [3H]cholesterol incorporation, by increasing the exchange rate, and the subsequent esterification of it irrespective of the source of exogenous [3H]cholesterol. The 7-oxocholesterol-stimulated esterification of exogenously derived LDL free [3H]cholesterol was progesterone-sensitive and energy-requiring.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6487273      PMCID： PMC1144247          DOI： 10.1042/bj2220821

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Inhibition of cholesteryl ester formation in human fibroblasts by an analogue of 7-ketocholesterol and by progesterone.

Authors:  J L Goldstein; J R Faust; J H Dygos; R J Chorvat; M S Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

2.  A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors:  M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

3.  Cholesterol ester formation in cultured human fibroblasts. Stimulation by oxygenated sterols.

Authors:  M S Brown; S E Dana; J L Goldstein
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

4.  Inhibition of cholesterol synthesis by oxygenated sterols.

Authors:  A A Kandutsch; H W Chen
Journal:  Lipids       Date:  1978-10       Impact factor: 1.880

5.  Consequences of blocked sterol synthesis in cultured cells. DNA synthesis and membrane composition.

Authors:  A A Kandutsch; H W Chen
Journal:  J Biol Chem       Date:  1977-01-25       Impact factor: 5.157

6.  Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

Authors:  W Gamble; M Vaughan; H S Kruth; J Avigan
Journal:  J Lipid Res       Date:  1978-11       Impact factor: 5.922

7.  Esterification of low density lipoprotein cholesterol in human fibroblasts and its absence in homozygous familial hypercholesterolemia.

Authors:  J L Goldstein; S E Dana; M S Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

8.  Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity.

Authors:  M S Brown; J L Goldstein
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

9.  Binding and degradation of low density lipoproteins by cultured human fibroblasts. Comparison of cells from a normal subject and from a patient with homozygous familial hypercholesterolemia.

Authors:  J L Goldstein; M S Brown
Journal:  J Biol Chem       Date:  1974-08-25       Impact factor: 5.157

10.  Regulation of cholesterol esterification and biosynthesis in monolayer cultures of normal adult rat hepatocytes.

Authors:  C A Drevon; D B Weinstein; D Steinberg
Journal:  J Biol Chem       Date:  1980-10-10       Impact factor: 5.157
View more
  3 in total

1.  In vivo regulation of human mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase. Decreased enzyme catalytic efficiency in familial hypercholesterolemia.

Authors:  P W Stacpoole; D M Bridge; I M Alvarez; R B Goldberg; H J Harwood
Journal:  J Clin Invest       Date:  1987-11       Impact factor: 14.808

2.  [3H]cholesterol transfer from microemulsion particles of different sizes to human fibroblasts.

Authors:  S Ekman
Journal:  Lipids       Date:  1987-09       Impact factor: 1.880

3.  Movement of plasma-membrane sterols to the endoplasmic reticulum in cultured cells.

Authors:  J P Slotte; E L Bierman
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.