Literature DB >> 673856

A method for the recovery of DNA from agarose gels.

H F Tabak, R A Flavell.   

Abstract

We describe a quick and versatile method for the isolation of DNA from agarose gels. The DNA is electrophoresed into a trough containing hydroxyapatite, where it is bound. The hydroxyapatite is taken out and the DNA eluted with phosphate buffer. By putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step. The DNA recovered can be used equally well in enzymatic incubations as DNA not purified through agarose gel electrophoresis. Several applications of this technique are described.

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Year:  1978        PMID： 673856      PMCID： PMC342166          DOI： 10.1093/nar/5.7.2321

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  The arrangement of simian virus 40 sequences in the DNA of transformed cells.

Authors:  M Botchan; W Topp; J Sambrook
Journal:  Cell       Date:  1976-10       Impact factor: 41.582

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.

Authors:  S M Tilghman; D C Tiemeier; F Polsky; M H Edgell; J G Seidman; A Leder; L W Enquist; B Norman; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

4.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

5.  Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

Authors:  K Danna; D Nathans
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

6.  Characterization of the agarolytic system of Agarbacterium pastinator.

Authors:  A R Sampietro; M A Vattuone de Sampietro
Journal:  Biochim Biophys Acta       Date:  1971-07-20

7.  Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.

Authors:  N Hozumi; S Tonegawa
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

8.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

9.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

10.  Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.

Authors:  U Pettersson; C Mulder; H Deluis; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205
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  112 in total

1.  Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.

Authors:  E O Long; I B Dawid
Journal:  Nucleic Acids Res       Date:  1979-09-11       Impact factor: 16.971

2.  Molecular analysis of chromosome-mediated gene transfer.

Authors:  G A Scangos; K M Huttner; S Silverstein; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

3.  Multiple cis-active elements in the long control region of bovine papillomavirus type 1 (BPV-1).

Authors:  S M Harrison; K L Gearing; S Y Kim; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

4.  The production of hybrid Ty:IFN virus-like particles in yeast.

Authors:  M H Malim; S E Adams; K Gull; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1987-09-25       Impact factor: 16.971

5.  Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens.

Authors:  M F Thomashow; R Nutter; K Postle; M D Chilton; F R Blattner; A Powell; M P Gordon; E W Nester
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

6.  Two conserved essential motifs of the murine immunoglobulin lambda enhancers bind B-cell-specific factors.

Authors:  C M Rudin; U Storb
Journal:  Mol Cell Biol       Date:  1992-01       Impact factor: 4.272

7.  Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

Authors:  H Horiuchi; M Takagi; K Yano
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

8.  Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.

Authors:  J Brennand; A C Chinault; D S Konecki; D W Melton; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

9.  DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning.

Authors:  K Harbers; A Schnieke; H Stuhlmann; D Jähner; R Jaenisch
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

10.  Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.

Authors:  D Shortle; P Novick; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205
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