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Literature DB >> 4567334

Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.

U Pettersson, C Mulder, H Deluis, P A Sharp.

Abstract

The DNA of adenovirus type 2 was cleaved by restriction endonuclease R.RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 x 10(6) to 13.6 x 10(6), as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.

Entities: Chemical Species

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Year: 1973 PMID： 4567334 PMCID： PMC433215 DOI： 10.1073/pnas.70.1.200

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.

Authors: M GREEN; M PINA
Journal: Virology Date: 1963-05 Impact factor: 3.616

2. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.

Authors: D Freifelder
Journal: J Mol Biol Date: 1970-12-28 Impact factor: 5.469

3. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

7. Heterogeneity of Epstein-Barr virus. III. Comparison of a transforming and a nontransforming virus by partial denaturation mapping of their DNAs.

Authors: H Delius; G W Bornkamm
Journal: J Virol Date: 1978-07 Impact factor: 5.103

Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.

1. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.

2. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.

3. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

4. Structural proteins of adenoviruses. I. Purification and characterization of the adenovirus type 2 hexon antigen.

5. Kinetics of renaturation of DNA.

6. Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopy.

7. DNA of bacteriophage PM2: a closed circular double-stranded molecule.

8. Adenovirus DNA. I. Molecular weight and conformation.

9. Structural proteins of adenoviruses. IV. Sequential degradation of the adenovirus type 2 virion.

10. Early events of virus-cell interaction in an adenovirus system.

1. Sites of cleavage by restriction enzymes in viral DNAs: comparison of observed and expected frequencies.

2. Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.

3. Study of the fine structure of adeno-associated virus DNA with bacterial restriction endonucleases.

4. Identification of early adenovirus type 2 RNA species transcribed from the left-hand end of the genome.

5. Termination sites for adenovirus type 2 DNA replication.

6. Differential accumulation of virus-specific RNA during the cell cycle of adenovirus-transformed rat embyro cells.

7. Heterogeneity of Epstein-Barr virus. III. Comparison of a transforming and a nontransforming virus by partial denaturation mapping of their DNAs.

8. State and organization of polyoma virus DNA sequences in transformed rat cell lines.

9. Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.

10. Biochemical studies on bovine adenovirus type 3. I. Purification and properties.