Literature DB >> 6952245

Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.

J Brennand, A C Chinault, D S Konecki, D W Melton, C T Caskey.   

Abstract

Cloned cDNA sequences of the murine hypoxanthine/guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) gene have been isolated by using a mouse neuroblastoma cell line containing increased levels of a variant HPRT protein. We have used these sequences as probes to demonstrate that protein overproduction in this cell line is a consequence of at least a 20-fold increase in HPRT mRNA levels resulting from approximately 50-fold amplification of HPRT genomic sequences. The largest cDNA insert so far characterized represents about 70% of the HPRT mRNA sequence. This cDNA is shown to possess regions of homology with mRNA and DNA from Chinese hamster, baboon, and human, thus facilitating detailed analysis of this locus in these four species.

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Year:  1982        PMID： 6952245      PMCID： PMC346099          DOI： 10.1073/pnas.79.6.1950

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  30 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  The isolation of a suppressible nonsense mutant in mammalian cells.

Authors:  M R Capecchi; R A Haar; N E Capecchi; M M Sveda
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

3.  Chinese hamster hypoxanthine-guanine phosphoribosyltransferase. Purification, structural, and catalytic properties.

Authors:  A S Olsen; G Milman
Journal:  J Biol Chem       Date:  1974-07-10       Impact factor: 5.157

4.  Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

Authors:  J D Sharp; N E Capecchi; M R Capecchi
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

5.  Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity.

Authors:  A Westerveld; R P Visser; M A Freeke; D Bootsma
Journal:  Biochem Genet       Date:  1972-08       Impact factor: 1.890

6.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

7.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688

8.  Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene.

Authors:  G M Wahl; S H Hughes; M R Capecchi
Journal:  J Cell Physiol       Date:  1975-04       Impact factor: 6.384

9.  Radioimmune determination of hypoxanthine phosphoribosyltransferase crossreacting material in erythrocytes of Lesch-Nyhan patients.

Authors:  G S Ghangas; G Milman
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

10.  Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.

Authors:  G Milman; S W Krauss; A S Olsen
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205
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  42 in total

1.  Lesch-Nyhan syndrome: molecular investigation of three French Canadian families using a hypoxanthine-guanine phosphoribosyltransferase cDNA probe.

Authors:  D Sinnett; L Lavergne; S B Melançon; L Dallaire; M Potier; D Labuda
Journal:  Hum Genet       Date:  1988-12       Impact factor: 4.132

Review 2.  Molecular genetics of the human X chromosome.

Authors:  K E Davies
Journal:  J Med Genet       Date:  1985-08       Impact factor: 6.318

3.  Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene.

Authors:  W F Flintoff; E Livingston; C Duff; R G Worton
Journal:  Mol Cell Biol       Date:  1984-01       Impact factor: 4.272

4.  Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.

Authors:  D W Melton; D S Konecki; J Brennand; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

5.  Toward a complete linkage map of the human X chromosome: regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids.

Authors:  P Wieacker; K E Davies; H J Cooke; P L Pearson; R Williamson; S Bhattacharya; J Zimmer; H H Ropers
Journal:  Am J Hum Genet       Date:  1984-03       Impact factor: 11.025

6.  Old and new genetics help ordering loci at the telomere of the human X-chromosome long arm.

Authors:  M Purrello; R Nussbaum; A Rinaldi; G Filippi; S Traccis; B Latte; M Siniscalco
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

7.  Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

Authors:  D S Konecki; J Brennand; J C Fuscoe; C T Caskey; A C Chinault
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

8.  Deletion and amplification of the HGPRT locus in Chinese hamster cells.

Authors:  J C Fuscoe; R G Fenwick; D H Ledbetter; C T Caskey
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272

9.  Molecular basis of hypoxanthine-guanine phosphoribosyltransferase deficiency in a patient with the Lesch-Nyhan syndrome.

Authors:  J M Wilson; W N Kelley
Journal:  J Clin Invest       Date:  1983-05       Impact factor: 14.808

10.  Amplification of hypoxanthine-guanine phosphoribosyltransferase genes in chromosome-mediated gene transferents.

Authors:  S Linder; A W Coleman; J M Eisenstadt
Journal:  Mol Cell Biol       Date:  1984-04       Impact factor: 4.272
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