Literature DB >> 6661185

Bile acid inhibition of basic and neutral glutathione S-transferases in rat liver.

J D Hayes, J Chalmers.   

Abstract

A purification scheme is described for the neutral glutathione S-transferases of rat liver. Discontinuous sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed that one of these enzymes contains a previously unidentified subunit, which has a molecular mass of 23 000 Da and has been designated Yn. Bile acids inhibited the activity of all the basic and neutral transferases investigated, but marked differences in the effects of bile acids on individual enzymes were observed. The activity of each transferase was inhibited more by lithocholate 3-sulphate than by chenodeoxycholate, which in turn was more inhibitory than cholate. The enzymes that were most sensitive to cholate inhibition were not found to be as readily inhibited as other transferases by chenodeoxycholate or lithocholate 3-sulphate. Conversely, the activity of transferase AA was more resistant to cholate, chenodeoxycholate and lithocholate 3-sulphate inhibition than was any of the other enzymes studied.

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Year:  1983        PMID： 6661185      PMCID： PMC1152439          DOI： 10.1042/bj2150581

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants.

Authors:  R C Strange; R Cramb; J D Hayes; I W Percy-Robb
Journal:  Biochem J       Date:  1977-09-01       Impact factor: 3.857

3.  The non-convalent binding of small molecules by ligandin. Interactions with steroids and their conjugates, fatty acids, bromosulphophthalein carcinogens, glutathione and realted compounds.

Authors:  E Tipping; B Ketterer; L Christodoulides; G Enderby
Journal:  Eur J Biochem       Date:  1976-08-16

4.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

Authors:  W H Habig; M J Pabst; W B Jakoby
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

5.  Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.

Authors:  G E Davies; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1970-07       Impact factor: 11.205

6.  A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors.

Authors:  A Cornish-Bowden
Journal:  Biochem J       Date:  1974-01       Impact factor: 3.857

7.  Cholic acid binding by glutathione S-transferases from rat liver cytosol.

Authors:  J D Hayes; R C Strange; I W Percy-Robb
Journal:  Biochem J       Date:  1980-01-01       Impact factor: 3.857

8.  Role of ligandin in transfer of bilirubin from plasma into liver.

Authors:  A W Wolkoff; C A Goresky; J Sellin; Z Gatmaitan; I M Arias
Journal:  Am J Physiol       Date:  1979-06

9.  Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions.

Authors:  A J Levi; Z Gatmaitan; I M Arias
Journal:  J Clin Invest       Date:  1969-11       Impact factor: 14.808

10.  Identification of two lithocholic acid-binding proteins. Separation of ligandin from glutathione S-transferase B.

Authors:  J D Hayes; R C Strange; I W Percy-Robb
Journal:  Biochem J       Date:  1979-09-01       Impact factor: 3.857
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  11 in total

1.  Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases.

Authors:  P Alin; H Jensson; E Cederlund; H Jörnvall; B Mannervik
Journal:  Biochem J       Date:  1989-07-15       Impact factor: 3.857

2.  Anomalous electrophoretic behaviour of the glutathione S-transferase Ya and Yk subunits isolated from man and rodents. A potential pitfall for nomenclature.

Authors:  J D Hayes; T J Mantle
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

3.  Use of immuno-blot techniques to discriminate between the glutathione S-transferase Yf, Yk, Ya, Yn/Yb and Yc subunits and to study their distribution in extrahepatic tissues. Evidence for three immunochemically distinct groups of transferase in the rat.

Authors:  J D Hayes; T J Mantle
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

4.  Purification and physical characterization of glutathione S-transferase K. Differential use of S-hexylglutathione and glutathione affinity matrices to isolate a novel glutathione S-transferase from rat liver.

Authors:  J D Hayes
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

5.  Suppression of high-affinity ligand binding to the major glutathione S-transferase from Galleria mellonella by physiological concentrations of glutathione.

Authors:  A G Clark; N Carrol
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857

6.  Purification and characterization of glutathione S-transferases P, S and N. Isolation from rat liver of Yb1 Yn protein, the existence of which was predicted by subunit hybridization in vitro.

Authors:  J D Hayes
Journal:  Biochem J       Date:  1984-12-15       Impact factor: 3.857

Review 7.  Role of gut in xenobiotic metabolism.

Authors:  O Hänninen; P Lindström-Seppä; K Pelkonen
Journal:  Arch Toxicol       Date:  1987       Impact factor: 5.153

8.  Identification of a basic hybrid glutathione S-transferase from human liver. Glutathione S-transferase delta is composed of two distinct subunits (B1 and B2).

Authors:  P K Stockman; G J Beckett; J D Hayes
Journal:  Biochem J       Date:  1985-04-15       Impact factor: 3.857

9.  Sex-specific constitutive expression of the pre-neoplastic marker glutathione S-transferase, YfYf, in mouse liver.

Authors:  L I McLellan; J D Hayes
Journal:  Biochem J       Date:  1987-07-15       Impact factor: 3.857

10.  Photoaffinity labelling of steroid-hormone-binding glutathione S-transferases with [3H]methyltrienolone. Inhibition of steroid-binding activity by the anticarcinogen indole-3-carbinol.

Authors:  D P Danger; W S Baldwin; G A LeBlanc
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857
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