Literature DB >> 6642321

Purification of DNA-dependent RNA polymerase from Streptomyces granaticolor.

J Spízek, P Rysavý, M Klégr, J Náprstek, J Janecek.   

Abstract

RNA nucleotidyltransferase (EC 2.7.7.6) of Streptomyces granaticolor was purified by precipitation with polymin P and ammonium sulphate, affinity chromatography on DNA- cellulose and gell filtration on Biogel A 1.5 m. SDS-polyacrylamide gel electrophoresis revealed 8 protein bands of molar mass ranging from 37 to 130 kg/mol. Proteins of molar mass of 130 and 120 kg/mol were identified to be beta and beta subunits, respectively. The role of other subunits of the enzyme is discussed.

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Year:  1983        PMID： 6642321     DOI： 10.1007/BF02879496

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  7 in total

1.  Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus.

Authors:  G H Jones
Journal:  Arch Biochem Biophys       Date:  1979-11       Impact factor: 4.013

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Differential staining of the subunits of DNA-dependent RNA polymerase.

Authors:  A M Rüger; W Rüger
Journal:  FEBS Lett       Date:  1980-11-03       Impact factor: 4.124

4.  Effect of rifampicin on in vitro RNA synthesis of Streptomyces mediterranei.

Authors:  S Watanabe; K Tanaka
Journal:  Biochem Biophys Res Commun       Date:  1976-09-20       Impact factor: 3.575

5.  Sensitivity of transcription by purified Streptomyces antibioticus RNA polymerase to actinomycin.

Authors:  G H Jones
Journal:  Biochem Biophys Res Commun       Date:  1978-10-30       Impact factor: 3.575

6.  Inhibitory effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells.

Authors:  M Saneyoshi; J Tohyama; C Nakayama; S Takiya; M Iwabuchi
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

7.  RNA synthesis in Streptomyces antibioticus: in vitro effects of actinomycin and transcriptional inhibitors from 48-h cells.

Authors:  G H Jones
Journal:  Biochemistry       Date:  1976-07-27       Impact factor: 3.162
  7 in total
  3 in total

1.  Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.

Authors:  M Krausová; M Klégr; J Spízek
Journal:  Folia Microbiol (Praha)       Date:  1994       Impact factor: 2.099

2.  Transformation of Streptomyces granaticolor with natural and recombinant plasmid vectors.

Authors:  M Petrícek; I Smrcková; P Tichý
Journal:  Folia Microbiol (Praha)       Date:  1985       Impact factor: 2.099

3.  Some properties of DNA-dependent RNA polymerase from Streptomyces granaticolor.

Authors:  P Rysavý; J Spízek; M Klégr; J Janecek; J Náprstek; J Houstĕk
Journal:  Folia Microbiol (Praha)       Date:  1985       Impact factor: 2.099
  3 in total

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