Literature DB >> 8181783

Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.

M Krausová1, M Klégr, J Spízek.   

Abstract

DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.

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Year:  1994        PMID： 8181783     DOI： 10.1007/bf02814521

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  18 in total

1.  Effect of protein phosphorylation on the activity of RNA polymerase in streptomycetes.

Authors:  J Smardová; Z Dobrová; M Havránek; J Janecek
Journal:  Folia Microbiol (Praha)       Date:  1992       Impact factor: 2.099

2.  Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to phage phi 29 DNA.

Authors:  J Smardová; J Felsberg; J Smarda; J Spízek
Journal:  Folia Microbiol (Praha)       Date:  1991       Impact factor: 2.099

3.  Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus.

Authors:  G H Jones
Journal:  Arch Biochem Biophys       Date:  1979-11       Impact factor: 4.013

4.  RNA polymerase heterogeneity in Streptomyces coelicolor.

Authors:  J Westpheling; M Ranes; R Losick
Journal:  Nature       Date:  1985 Jan 3-9       Impact factor: 49.962

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors:  R R Burgess; J J Jendrisak
Journal:  Biochemistry       Date:  1975-10-21       Impact factor: 3.162

7.  Purification of DNA-dependent RNA polymerase from Streptomyces granaticolor.

Authors:  J Spízek; P Rysavý; M Klégr; J Náprstek; J Janecek
Journal:  Folia Microbiol (Praha)       Date:  1983       Impact factor: 2.099

8.  Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans.

Authors:  M Petrícek; K Stajner; P Tichý
Journal:  J Gen Microbiol       Date:  1989-12

9.  Purification of Bacillus subtilis RNA polymerase with heparin-agarose. In vitro transcription of phi 29 DNA.

Authors:  B L Davison; T Leighton; J C Rabinowitz
Journal:  J Biol Chem       Date:  1979-09-25       Impact factor: 5.157

10.  Inhibitory effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells.

Authors:  M Saneyoshi; J Tohyama; C Nakayama; S Takiya; M Iwabuchi
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971
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