Literature DB >> 3936758

Some properties of DNA-dependent RNA polymerase from Streptomyces granaticolor.

P Rysavý, J Spízek, M Klégr, J Janecek, J Náprstek, J Houstĕk.   

Abstract

RNA nucleotidyltransferase from Streptomyces granaticolor was purified and some of its properties were investigated. The temperature optimum of the enzyme is 35 degrees C, pH optimum 7.8-8.4. Antibodies against the beta subunit of the enzyme from Bacillus subtilis immunologically cross-react with the beta subunit of the enzyme from S. granaticolor. Antibodies against the beta' subunit of the enzyme from B. subtilis immunologically cross-react with both beta and beta' subunits of the enzyme from S. granaticolor.

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Year:  1985        PMID： 3936758     DOI： 10.1007/bf02927616

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  8 in total

1.  Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus.

Authors:  G H Jones
Journal:  Arch Biochem Biophys       Date:  1979-11       Impact factor: 4.013

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Differential staining of the subunits of DNA-dependent RNA polymerase.

Authors:  A M Rüger; W Rüger
Journal:  FEBS Lett       Date:  1980-11-03       Impact factor: 4.124

5.  Purification of DNA-dependent RNA polymerase from Streptomyces granaticolor.

Authors:  J Spízek; P Rysavý; M Klégr; J Náprstek; J Janecek
Journal:  Folia Microbiol (Praha)       Date:  1983       Impact factor: 2.099

Review 6.  Multiple RNA polymerase holoenzymes exert transcriptional specificity in Bacillus subtilis.

Authors:  R H Doi
Journal:  Arch Biochem Biophys       Date:  1982-04-01       Impact factor: 4.013

7.  The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes.

Authors:  B Batteiger; W J Newhall; R B Jones
Journal:  J Immunol Methods       Date:  1982-12-30       Impact factor: 2.303

8.  Inhibitory effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells.

Authors:  M Saneyoshi; J Tohyama; C Nakayama; S Takiya; M Iwabuchi
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971
  8 in total
  2 in total

1.  Effect of granaticin on the activity of DNA-directed RNA polymerase from Streptomyces granaticolor.

Authors:  J Smardová; J Spízek; J Stastná; M Klégr
Journal:  Folia Microbiol (Praha)       Date:  1989       Impact factor: 2.099

2.  Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.

Authors:  M Krausová; M Klégr; J Spízek
Journal:  Folia Microbiol (Praha)       Date:  1994       Impact factor: 2.099
  2 in total

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