| Literature DB >> 3002927 |
M Petrícek, I Smrcková, P Tichý.
Abstract
Protoplasts of Streptomyces granaticolor were found to be transformable by the broad-host-range plasmid pIJ350 but no transformants were detected when the narrow-host-range plasmid pIJ2 or the shuttle vector pPM66 (pIJ350--pBR322) isolated from E. coli cells were used. The onset of blue colour granaticin production by S. granaticolor cells was used as a marker to prepare protoplasts with a high transformation capacity. The presence of a restriction system is discussed.Entities:
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Year: 1985 PMID: 3002927 DOI: 10.1007/bf02927609
Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN: 0015-5632 Impact factor: 2.099