Literature DB >> 6630448

Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.

B M Madison, V S Baselski.   

Abstract

The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonuclease test provides a rapid, reliable method to identify S. aureus in blood cultures.

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Year:  1983        PMID： 6630448      PMCID： PMC270883          DOI： 10.1128/jcm.18.3.722-724.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests.

Authors:  A L Barry; R V Lachica; F W Atchison
Journal:  Appl Microbiol       Date:  1973-03

2.  Evaluation of positive blood cultures. Guidelines for early differentiation of contaminated from valid positive cultures.

Authors:  R R MacGregor; H N Beaty
Journal:  Arch Intern Med       Date:  1972-07

3.  Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

Authors:  R V Lachica; C Genigeorgis; P D Hoeprich
Journal:  Appl Microbiol       Date:  1971-04

4.  Efficacy of direct Gram stain in differentiating staphylococci from streptococci in blood cultures positive for gram-positive cocci.

Authors:  W A Agger; D G Maki
Journal:  J Clin Microbiol       Date:  1978-02       Impact factor: 5.948

5.  Metachromatic agar-diffusion microslide technique for detecting staphylococcal nuclease in foods.

Authors:  R V Lachica; P D Hoeprich; C Genigeorgis
Journal:  Appl Microbiol       Date:  1972-01

6.  Presumptive identification of bacteria from blood cultures in four hours.

Authors:  B L Wasilauskas; P D Ellner
Journal:  J Infect Dis       Date:  1971-11       Impact factor: 5.226

7.  Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus.

Authors:  M K Rayman; C E Park; J Philpott; E C Todd
Journal:  Appl Microbiol       Date:  1975-04

8.  Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus.

Authors:  R E Menzies
Journal:  J Clin Pathol       Date:  1977-07       Impact factor: 3.411

9.  Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources.

Authors:  J Y Zarzour; E A Belle
Journal:  J Clin Microbiol       Date:  1978-02       Impact factor: 5.948

10.  Production and heat stability of staphylococcal nuclease.

Authors:  A Erickson; R H Deibel
Journal:  Appl Microbiol       Date:  1973-03
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  13 in total

Review 1.  Update on detection of bacteremia and fungemia.

Authors:  L G Reimer; M L Wilson; M P Weinstein
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

2.  Thermostable DNase is superior to tube coagulase for direct detection of Staphylococcus aureus in positive blood cultures.

Authors:  Philippe R S Lagacé-Wiens; Michelle J Alfa; Kanchana Manickam; James A Karlowsky
Journal:  J Clin Microbiol       Date:  2007-08-08       Impact factor: 5.948

3.  Problems of thermonuclease detection for identifying Staphylococcus aureus in blood culture broths.

Authors:  G M Megson; D Law; L A Ganguli
Journal:  J Clin Pathol       Date:  1991-09       Impact factor: 3.411

4.  Medium dependence for rapid detection of thermonuclease activity in blood culture broths.

Authors:  H Faruki; P Murray
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

5.  Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp.

Authors:  Sukanya Sudhaharan; Lavanya Vanjari; Neeraja Mamidi; Nagapriyanka Ede; Lakshmi Vemu
Journal:  J Clin Diagn Res       Date:  2015-08-01

6.  Rapid detection of oxacillin-resistant Staphylococcus aureus in blood cultures by an impedance method.

Authors:  J J Wu; A H Huang; J H Dai; T C Chang
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

7.  Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.

Authors:  O G Brakstad; K Aasbakk; J A Maeland
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

8.  Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi.

Authors:  Feng Li Yang; Xiao Shan Li; Xian Wei Liang; Xiu Fang Zhang; Guang Sheng Qin; Bing Zhuang Yang
Journal:  Trop Anim Health Prod       Date:  2012-04-14       Impact factor: 1.559

9.  Thermonuclease test for same-day identification of Staphylococcus aureus in blood cultures.

Authors:  H B Ratner; C W Stratton
Journal:  J Clin Microbiol       Date:  1985-06       Impact factor: 5.948

10.  Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible S. aureus Directly from Positive Blood Cultures by Use of the BD Max StaphSR Assay.

Authors:  Justin A Ellem; Tom Olma; Matthew V N O'Sullivan
Journal:  J Clin Microbiol       Date:  2015-12       Impact factor: 5.948
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