Literature DB >> 4930272

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

R V Lachica, C Genigeorgis, P D Hoeprich.   

Abstract

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

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Year:  1971        PMID： 4930272      PMCID： PMC377235          DOI： 10.1128/am.21.4.585-587.1971

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  5 in total

1.  The structure of the DNA-acridine complex.

Authors:  L S LERMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1963-01-15       Impact factor: 11.205

2.  Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.

Authors:  P Cuatrecasas; S Fuchs; C B Anfinsen
Journal:  J Biol Chem       Date:  1967-04-10       Impact factor: 5.157

3.  Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.

Authors:  R Victor; F Lachica; K F Weiss; R H Deibel
Journal:  Appl Microbiol       Date:  1969-07

4.  Detection of nuclease activity in semisolid and broth cultures.

Authors:  R V Lachica; R H Deibel
Journal:  Appl Microbiol       Date:  1969-08

5.  Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.

Authors:  M M STREITFELD; E M HOFFMANN; H M JANKLOW
Journal:  J Bacteriol       Date:  1962-07       Impact factor: 3.490
  5 in total
  106 in total

1.  DNases in milk and blood sera from different species.

Authors:  R Gudding
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2.  Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius.

Authors:  O Chesneau; N el Solh
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

3.  Synthesis of Escherichia coli heat-stable enterotoxin STp as a pre-pro form and role of the pro sequence in secretion.

Authors:  K Okamoto; M Takahara
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

4.  A method for monitoring antibodies against staphylococcal DNases.

Authors:  S Høie; R Gudding
Journal:  Acta Vet Scand       Date:  1990       Impact factor: 1.695

5.  Construction of a database to identify Staphylococcus species.

Authors:  C Geary; M Stevens; P H Sneath; C J Mitchell
Journal:  J Clin Pathol       Date:  1989-03       Impact factor: 3.411

6.  Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations.

Authors:  D Shortle; B Lin
Journal:  Genetics       Date:  1985-08       Impact factor: 4.562

7.  Rapid identification of Capnocytophaga isolated from septicemic patients.

Authors:  J E Kristiansen; A Bremmelgaard; H E Busk; O Heltberg; W Frederiksen; T Justesen
Journal:  Eur J Clin Microbiol       Date:  1984-06       Impact factor: 3.267

8.  Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus.

Authors:  R E Menzies
Journal:  J Clin Pathol       Date:  1977-07       Impact factor: 3.411

9.  Phenotypic distinctiveness of Staphylococcus aureus strains associated with toxic shock syndrome.

Authors:  J K Todd; A Franco-Buff; D W Lawellin; M L Vasil
Journal:  Infect Immun       Date:  1984-08       Impact factor: 3.441

10.  Comparison of rapid identification assays for Staphylococcus aureus.

Authors:  K E Aldridge; C Kogos; C V Sanders; R L Marier
Journal:  J Clin Microbiol       Date:  1984-05       Impact factor: 5.948
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