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Literature DB >> 4698860

Production and heat stability of staphylococcal nuclease.

Abstract

No correlation existed between numbers of organisms and nuclease activity in laboratory-grown cultures of Staphylococcus aureus. Nuclease production was inhibited by anaerobic incubation and stimulated by aeration. Strains of S. aureus varied in the production of nuclease. The optimum pH for enzyme production was 8.3 and employment of a tris(hydroxymethyl)aminomethane buffer system resulted in increased production of the enzyme as compared with a phosphate buffer. The nuclease was extremely heat-stable and had a D value of 16.6 min at 130 C.

Entities: Chemical Species

Mesh：

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Year: 1973 PMID： 4698860 PMCID： PMC380806 DOI： 10.1128/am.25.3.332-336.1973

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

5 in total

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Authors: C D JEFFRIES; D F HOLTMAN; D G GUSE
Journal: J Bacteriol Date: 1957-04 Impact factor: 3.490

2. Turbidimetric assay of staphylococcal nuclease.

Authors: A Erickson; R H Deibel
Journal: Appl Microbiol Date: 1973-03

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Authors: P B Smith; G A Hancock; D L Rhoden
Journal: Appl Microbiol Date: 1969-12

4. Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.

Authors: R Victor; F Lachica; K F Weiss; R H Deibel
Journal: Appl Microbiol Date: 1969-07

5. Enzymatic detection of the growth of Staphylococcus aureus in foods.

Authors: W R Chesbro; K Auborn
Journal: Appl Microbiol Date: 1967-09

5 in total

12 in total

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Authors: R Gudding
Journal: Acta Vet Scand Date: 1979 Impact factor: 1.695

2. Predicting extreme pKa shifts in staphylococcal nuclease mutants with constant pH molecular dynamics.

Authors: Evan J Arthur; Joseph D Yesselman; Charles L Brooks
Journal: Proteins Date: 2011-10-15

Review 3. At the crossroads of bacterial metabolism and virulence factor synthesis in Staphylococci.

Authors: Greg A Somerville; Richard A Proctor
Journal: Microbiol Mol Biol Rev Date: 2009-06 Impact factor: 11.056

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Authors: A Niskanen; E Nurmi
Journal: Appl Environ Microbiol Date: 1976-01 Impact factor: 4.792

5. The demonstration and characterization of deoxyribonucleases of streptococci group A, B, C, G and L.

Authors: R Gudding
Journal: Acta Vet Scand Date: 1979 Impact factor: 1.695

6. Medium dependence for rapid detection of thermonuclease activity in blood culture broths.

Authors: H Faruki; P Murray
Journal: J Clin Microbiol Date: 1986-09 Impact factor: 5.948

7. Nuclease of staphylococcus epidermidis isolated from mastitic milk. Production and some properties.

Authors: R Gudding
Journal: Acta Vet Scand Date: 1980 Impact factor: 1.695

8. Rapid detection of staphylococcal thermonuclease on casings of naturally contaminated fermented sausages.

Authors: B S Emswiler-Rose; R W Johnston; M E Harris; W H Lee
Journal: Appl Environ Microbiol Date: 1980-07 Impact factor: 4.792

9. Differentiation of staphylococci on the basis of nuclease properties.

Authors: R Gudding
Journal: J Clin Microbiol Date: 1983-11 Impact factor: 5.948

10. Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.

Authors: B M Madison; V S Baselski
Journal: J Clin Microbiol Date: 1983-09 Impact factor: 5.948