Literature DB >> 6585346

A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids.

H F Willard, M T Holmes.   

Abstract

A radioisotopic assay is described to distinguish between Chinese hamster and human steroid sulfatase activity in extracts prepared from hamster X human somatic cell hybrids. This assay is based on different pH optima and provides a sensitive and unambiguous biochemical marker for the short arm of the human X chromosome and as well as for otherwise genetically inactivated X chromosomes in rodent X human hybrids.

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Year:  1984        PMID: 6585346     DOI: 10.1007/bf00286615

Source DB:  PubMed          Journal:  Hum Genet        ISSN: 0340-6717            Impact factor:   4.132


  17 in total

1.  Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase.

Authors:  R S Sparkes; M C Baluda; D E Townsend
Journal:  J Lab Clin Med       Date:  1969-03

2.  X-linkage of human -galactosidase.

Authors:  K H Grzeschik; A M Grzeschik; S Banhof; G Romeo; M Siniscalco; H van Someren; P Meera Khan; A Westerveld; D Bootsma
Journal:  Nat New Biol       Date:  1972-11-08

3.  Isolation and characterization of a major tandem repeat family from the human X chromosome.

Authors:  H F Willard; K D Smith; J Sutherland
Journal:  Nucleic Acids Res       Date:  1983-04-11       Impact factor: 16.971

4.  Quantitative analysis of high-resolution trypsin-giemsa bands on human prometaphase chromosomes.

Authors:  U Francke; N Oliver
Journal:  Hum Genet       Date:  1978-12-18       Impact factor: 4.132

5.  Identification of an autosomal locus affecting steroid sulfatase activity among inbred strains of mice.

Authors:  R P Erickson; K Harper; J M Kramer
Journal:  Genetics       Date:  1983-09       Impact factor: 4.562

6.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

Authors:  R L Davidson; P S Gerald
Journal:  Somatic Cell Genet       Date:  1976-03

7.  Evidence for X-linkage of steroid sulfatase in the mouse: steroid sulfatase levels in oocytes of XX and XO mice.

Authors:  S M Gartler; M Rivest
Journal:  Genetics       Date:  1983-01       Impact factor: 4.562

8.  Regional assignment of the steroid sulfatase-X-linked ichthyosis locus: implications for a noninactivated region on the short arm of human X chromosome.

Authors:  T Mohandas; L J Shapiro; R S Sparkes; M C Sparkes
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

9.  Clinical and biochemical investigations on patients with partial deficiency of placental steroid sulfatase.

Authors:  H Hameister; G Wolff; C H Lauritzen; W O Lehmann; A Hauser; H H Ropers
Journal:  Hum Genet       Date:  1979-01-25       Impact factor: 4.132

10.  Expression of an X-linked gene from an inactive human X chromosome in mouse-human hybrid cells: further evidence for the noninactivation of the steroid sulfatase locus in man.

Authors:  T Mohandas; R S Sparkes; B Hellkuhl; K H Grzeschik; L J Shapiro
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

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  12 in total

1.  Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement.

Authors:  R Wevrick; W C Earnshaw; P N Howard-Peebles; H F Willard
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

2.  Organization and genomic distribution of "82H" alpha satellite DNA. Evidence for a low-copy or single-copy alphoid domain located on human chromosome 14.

Authors:  J S Waye; A R Mitchell; H F Willard
Journal:  Hum Genet       Date:  1988-01       Impact factor: 4.132

3.  Chromosome-specific organization of human alpha satellite DNA.

Authors:  H F Willard
Journal:  Am J Hum Genet       Date:  1985-05       Impact factor: 11.025

4.  Regional localization of the phosphoglycerate kinase gene and pseudogene on the human X chromosome and assignment of a related DNA sequence to chromosome 19.

Authors:  H F Willard; S J Goss; M T Holmes; D L Munroe
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

5.  Isolation of cDNA clones coding for the beta subunit of human beta-hexosaminidase.

Authors:  B F O'Dowd; F Quan; H F Willard; A M Lamhonwah; R G Korneluk; J A Lowden; R A Gravel; D J Mahuran
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

6.  Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome.

Authors:  J S Waye; S B England; H F Willard
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

7.  The breakpoint of an inversion of chromosome 14 in a T-cell leukemia: sequences downstream of the immunoglobulin heavy chain locus are implicated in tumorigenesis.

Authors:  R Baer; A Heppell; A M Taylor; P H Rabbitts; B Boullier; T H Rabbitts
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

8.  Molecular cloning and chromosomal localization of DNA sequences associated with a human DNA repair gene.

Authors:  J S Rubin; V R Prideaux; H F Willard; A M Dulhanty; G F Whitmore; A Bernstein
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

9.  Direct regional assignment of the gene for vitamin D binding protein (Gc-globulin) to human chromosome 4q11-q13 and identification of an associated DNA polymorphism.

Authors:  N E Cooke; H F Willard; E V David; D L George
Journal:  Hum Genet       Date:  1986-07       Impact factor: 4.132

10.  Noninactivation of a selectable human X-linked gene that complements a murine temperature-sensitive cell cycle defect.

Authors:  C J Brown; H F Willard
Journal:  Am J Hum Genet       Date:  1989-10       Impact factor: 11.025

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