Literature DB >> 6549064

Synthesis of phosphorothioate-containing DNA fragments by a modified hydroxybenzotriazole phosphotriester approach.

J E Marugg, C van den Bergh, M Tromp, G A van der Marel, W J van Zoest, J H van Boom.   

Abstract

The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.

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Year:  1984        PMID： 6549064      PMCID： PMC320440          DOI： 10.1093/nar/12.23.9095

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Absolute configuration of the diastereomers of adenosine 5'-O-(1-thiotriphosphate): consequences for the stereochemistry of polymerization by DNA-dependent RNA polymerase from Escherichia coli.

Authors:  P M Burgers; F Eckstein
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

2.  Molecular structure of a left-handed double helical DNA fragment at atomic resolution.

Authors:  A H Wang; G J Quigley; F J Kolpak; J L Crawford; J H van Boom; G van der Marel; A Rich
Journal:  Nature       Date:  1979-12-13       Impact factor: 49.962

3.  Stereochemical course of polymerization catalyzed by avian myeloblastosis virus reverse transcriptase.

Authors:  P A Bartlett; F Eckstein
Journal:  J Biol Chem       Date:  1982-08-10       Impact factor: 5.157

4.  Molecular structure of (m5 dC-dG)3: the role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA.

Authors:  S Fujii; A H Wang; G van der Marel; J H van Boom; A Rich
Journal:  Nucleic Acids Res       Date:  1982-12-11       Impact factor: 16.971

5.  Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site.

Authors:  B A Connolly; B V Potter; F Eckstein; A Pingoud; L Grotjahn
Journal:  Biochemistry       Date:  1984-07-17       Impact factor: 3.162

6.  Left-handed double helical DNA: variations in the backbone conformation.

Authors:  A J Wang; G J Quigley; F J Kolpak; G van der Marel; J H van Boom; A Rich
Journal:  Science       Date:  1981-01-09       Impact factor: 47.728

7.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

8.  Stereochemistry of internucleotide bond formation by polynucleotide phosphorylase from Micrococcus luteus.

Authors:  P M Burgers; F Eckstein
Journal:  Biochemistry       Date:  1979-02-06       Impact factor: 3.162

9.  Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors:  C B Reese; L Zard
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

10.  Synthesis and configurational analysis of a dinucleoside phosphate isotopically chiral at phosphorus. Stereochemical course of Penicillium citrum nuclease P1 reaction.

Authors:  B V Potter; B A Connolly; F Eckstein
Journal:  Biochemistry       Date:  1983-03-15       Impact factor: 3.162
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  4 in total

1.  Phosphorothioate-modified oligodeoxyribonucleotides. III. NMR and UV spectroscopic studies of the Rp-Rp, Sp-Sp, and Rp-Sp duplexes, [d(GGSAATTCC)]2, derived from diastereomeric O-ethyl phosphorothioates.

Authors:  L A LaPlanche; T L James; C Powell; W D Wilson; B Uznanski; W J Stec; M F Summers; G Zon
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971

2.  Synthesis of nucleic acid methylphosphonothioates.

Authors:  H C Roelen; E de Vroom; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

3.  An approach to the stereoselective synthesis of Sp-dinucleoside phosphorothioates using phosphotriester chemistry.

Authors:  R Cosstick; D M Williams
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

4.  Synthesis of nucleic acid methylphosphonates via the 1-hydroxybenzotriazole phosphotriester approach.

Authors:  J E Marugg; E de Vroom; C E Dreef; M Tromp; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971
  4 in total

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