Literature DB >> 6420394

Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.

D K Leith, J B Baseman.   

Abstract

A 165,000-dalton surface protein of Mycoplasma pneumoniae, designated protein P1, appears to be the major attachment ligand of the pathogen. We employed monoclonal antibody affinity chromatography to obtain purified protein P1.

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Year:  1984        PMID： 6420394      PMCID： PMC215303          DOI： 10.1128/jb.157.2.678-680.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

1.  The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.

Authors:  K K Andersson; Y Benyamin; P Douzou; C Balny
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

2.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

3.  Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains.

Authors:  R P Lipman; W A Clyde; F W Denny
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490

4.  Tissue cultures and mycoplasmas.

Authors:  L Hayflick
Journal:  Tex Rep Biol Med       Date:  1965-06

5.  An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae.

Authors:  J Morrison-Plummer; D H Jones; J B Baseman
Journal:  J Immunol Methods       Date:  1983-11-11       Impact factor: 2.303

6.  Mycoplasma pneumoniae proteins that selectively bind to host cells.

Authors:  D C Krause; J B Baseman
Journal:  Infect Immun       Date:  1982-07       Impact factor: 3.441

7.  Isolation of binding sites to glycophorin from Mycoplasma pneumoniae membranes.

Authors:  M Banai; S Razin; W Bredt; I Kahane
Journal:  Infect Immun       Date:  1980-12       Impact factor: 3.441

8.  Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody.

Authors:  J Feldner; U Göbel; W Bredt
Journal:  Nature       Date:  1982-08-19       Impact factor: 49.962

9.  Molecular basis for cytadsorption of Mycoplasma pneumoniae.

Authors:  J B Baseman; R M Cole; D C Krause; D K Leith
Journal:  J Bacteriol       Date:  1982-09       Impact factor: 3.490

10.  Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium.

Authors:  P C Hu; A M Collier; J B Baseman
Journal:  J Exp Med       Date:  1977-05-01       Impact factor: 14.307
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  6 in total

1.  Evidence for Separate Adhesion Mechanisms for Hydrophilic and Hydrophobic Surfaces in Vibrio proteolytica.

Authors:  J H Paul; W H Jeffrey
Journal:  Appl Environ Microbiol       Date:  1985-08       Impact factor: 4.792

2.  A 168-kilodalton protein of Mycoplasma pneumoniae used as antigen in a dot enzyme-linked immunosorbent assay.

Authors:  E Jacobs; K Fuchte; W Bredt
Journal:  Eur J Clin Microbiol       Date:  1986-08       Impact factor: 3.267

3.  Detection of the major adhesin P1 in triton shells of virulent Mycoplasma pneumoniae.

Authors:  I Kahane; S Tucker; D K Leith; J Morrison-Plummer; J B Baseman
Journal:  Infect Immun       Date:  1985-12       Impact factor: 3.441

4.  Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae.

Authors:  C J Su; V V Tryon; J B Baseman
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

5.  Adherence inhibition assay: a specific serological test for detection of antibodies to Mycoplasma pneumoniae.

Authors:  E Jacobs; K Schöpperle; W Bredt
Journal:  Eur J Clin Microbiol       Date:  1985-04       Impact factor: 3.267

6.  Detection of Mycoplasma pneumoniae adhesin (P1) in the nonhemadsorbing population of virulent Mycoplasma pneumoniae.

Authors:  I Kahane; S Tucker; J B Baseman
Journal:  Infect Immun       Date:  1985-08       Impact factor: 3.441
  6 in total

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