Literature DB >> 6196412

An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae.

J Morrison-Plummer, D H Jones, J B Baseman.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for analysis of monoclonal antibodies directed towards lipid determinants of Mycoplasma pneumoniae. Chloroform-methanol lipid extracts as well as chromatographed lipid fractions of M. pneumoniae were bound to polyvinylchloride microtiter wells. Of 293 clones positive to M. pneumoniae as detected by a whole cell ELISA, a total of 78 clones produced antibodies which selectively bound to lipid extracts of M. pneumoniae. The simplicity and reproducibility of the assay permit rapid screening for detection of antibodies directed against non-protein cellular antigens.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6196412     DOI： 10.1016/0022-1759(83)90395-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  11 in total

1.  Preparation of Mycoplasma hyopneumoniae antigen for the enzyme-linked immunosorbent assay.

Authors:  S Kazama; T Yagihashi; K Seto
Journal:  Can J Vet Res       Date:  1989-04       Impact factor: 1.310

2.  Species-specific monoclonal antibody to a 43,000-molecular-weight membrane protein of Mycoplasma pneumoniae.

Authors:  R D Madsen; F A Saeed; O Gray; B M Fendly; S R Coates
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

3.  Immunological cross-reactivity of a Mycoplasma pneumoniae membrane-associated protein antigen with Mycoplasma genitalium and Acholeplasma laidlawii.

Authors:  N Cimolai; L E Bryan; M To; D E Woods
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

4.  Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae.

Authors:  J Morrison-Plummer; D K Leith; J B Baseman
Journal:  Infect Immun       Date:  1986-08       Impact factor: 3.441

5.  Detection of the major adhesin P1 in triton shells of virulent Mycoplasma pneumoniae.

Authors:  I Kahane; S Tucker; D K Leith; J Morrison-Plummer; J B Baseman
Journal:  Infect Immun       Date:  1985-12       Impact factor: 3.441

6.  Monoclonal antibodies to Treponema pallidum: recognition of a major polypeptide antigen.

Authors:  R W Thornburg; J Morrison-Plummer; J B Baseman
Journal:  Genitourin Med       Date:  1985-02

7.  Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.

Authors:  D K Leith; J B Baseman
Journal:  J Bacteriol       Date:  1984-02       Impact factor: 3.490

8.  Absence of Mycoplasma pneumoniae cytadsorption protein P1 in Mycoplasma genitalium and Mycoplasma gallisepticum.

Authors:  J B Baseman; D L Drouillard; D K Leith; J G Tully
Journal:  Infect Immun       Date:  1984-03       Impact factor: 3.441

9.  Monoclonal antibody to a major surface glycoprotein immunogen differentiates isolates and subpopulations of Trichomonas vaginalis.

Authors:  J F Alderete; L Suprun-Brown; L Kasmala
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

10.  Biological activities of monoclonal antibodies to Mycoplasma pneumoniae membrane glycolipids.

Authors:  D K Chandler; L D Olson; J G Kenimer; P G Probst; S Rottem; M W Grabowski; M F Barile
Journal:  Infect Immun       Date:  1989-04       Impact factor: 3.441
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.