| Literature DB >> 372447 |
K K Andersson, Y Benyamin, P Douzou, C Balny.
Abstract
Physicochemical parameters governing the elution of yeast 3-phosphoglycerate kinase from its immunoadsorbent were studied. Non-denaturing elution conditions were determined (alkali medium containing 50%, v/v, ethylene glycol) and the method was applied to one-step isolation of enzyme from a crude yeast preparation.Entities:
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Year: 1979 PMID： 372447 DOI： 10.1016/0022-1759(79)90029-2
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303