Literature DB >> 6359064

Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.

B H Pheiffer, S B Zimmerman.   

Abstract

The rates of blunt-end and cohesive-end ligation of DNA by T4 DNA ligase are increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, Ficoll, bovine plasma albumin, or glycogen. Blunt-end ligation of small self-complementary oligodeoxyribonucleotides is also stimulated. The use of polyethylene glycol 6000 in such systems is characterized in some detail. Conditions are suggested which either greatly increase the rate of formation of and size of linear ligation products or which allow smaller but significant improvements in the amounts of circular ligation products.

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Year:  1983        PMID： 6359064      PMCID： PMC326544          DOI： 10.1093/nar/11.22.7853

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

3.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

4.  Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H.

Authors:  R J Roberts; G A Wilson; F E Young
Journal:  Nature       Date:  1977-01-06       Impact factor: 49.962

5.  Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine.

Authors:  M A Krasnow; N R Cozzarelli
Journal:  J Biol Chem       Date:  1982-03-10       Impact factor: 5.157

6.  Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.

Authors:  S B Zimmerman; B H Pheiffer
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

7.  T4 polynucleotide ligase catalyzed joining of short synthetic DNA duplexes at base-paired ends.

Authors:  K V Deugau; J H van de Sande
Journal:  Biochemistry       Date:  1978-02-21       Impact factor: 3.162

8.  Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends.

Authors:  A Sugino; H M Goodman; H L Heyneker; J Shine; H W Boyer; N R Cozzarelli
Journal:  J Biol Chem       Date:  1977-06-10       Impact factor: 5.157
  8 in total
  42 in total

1.  Construction and analysis of a library for random insertional mutagenesis in Streptococcus pneumoniae: use for recovery of mutants defective in genetic transformation and for identification of essential genes.

Authors:  M S Lee; B A Dougherty; A C Madeo; D A Morrison
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  Increasing the efficiency of SAGE adaptor ligation by directed ligation chemistry.

Authors:  Austin P So; Robin F B Turner; Charles A Haynes
Journal:  Nucleic Acids Res       Date:  2004-07-06       Impact factor: 16.971

3.  Surface-directed DNA condensation in the absence of soluble multivalent cations.

Authors:  Y Fang; J H Hoh
Journal:  Nucleic Acids Res       Date:  1998-01-15       Impact factor: 16.971

4.  Blunt-ended ligation can be used to produce DNA ladders with rung spacing as large as 0.17 Mb.

Authors:  D Louie; P Serwer
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

5.  Efficient RecABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene.

Authors:  W C Hollifield; E N Kaplan; H V Huang
Journal:  Mol Gen Genet       Date:  1987-12

6.  Macromolecular crowding accelerates the cohesion of DNA fragments with complementary termini.

Authors:  S B Zimmerman; B Harrison
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

7.  Purification of the gam gene-product of bacteriophage Mu and determination of the nucleotide sequence of the gam gene.

Authors:  J E Akroyd; E Clayson; N P Higgins
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971

8.  Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A.

Authors:  P J Nielsen; G K McMaster; H Trachsel
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

9.  Mutations in the adenovirus major late promoter: effects on viability and transcription during infection.

Authors:  L J Brunet; L E Babiss; C S Young; D R Mills
Journal:  Mol Cell Biol       Date:  1987-03       Impact factor: 4.272

10.  Construction of a circular single-stranded DNA template containing a defined lesion.

Authors:  Kiyonobu Karata; Antonio E Vidal; Roger Woodgate
Journal:  DNA Repair (Amst)       Date:  2009-04-21
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