Literature DB >> 3840589

Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A.

P J Nielsen, G K McMaster, H Trachsel.   

Abstract

Monoclonal antibodies directed against rabbit reticulocyte protein synthesis initiation factor 4A (eIF-4A) were used to isolate mouse cDNA clones expressing eIF-4A protein sequences in E. coli. The identity of cDNA clones encoding eIF-4A sequences was confirmed by hybrid-selected translation and peptide mapping of the translation product. Analysis of the mRNA coding for eIF-4A from mouse liver and HeLa cells by Northern hybridization revealed two discrete mRNA species of approximately 2000 and 1600 nucleotides in length. The existence of two mRNAs in mouse and HeLa cells encoding eIF-4A was confirmed by cDNA sequencing.

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Year:  1985        PMID： 3840589      PMCID： PMC322010          DOI： 10.1093/nar/13.19.6867

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  30 in total

1.  Purification and characterization of initiation factors IF-E4 and IF-E6 from rabbit reticulocytes.

Authors:  R Benne; M Luedi; J W Hershey
Journal:  J Biol Chem       Date:  1977-08-25       Impact factor: 5.157

Review 2.  The molecular mechanism of hemoglobin synthesis and its regulation in the reticulocyte.

Authors:  B Safer; W F Anderson
Journal:  CRC Crit Rev Biochem       Date:  1978

Review 3.  Post-transcriptional and translational controls of gene expression in eukaryotes.

Authors:  M Revel; Y Groner
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

4.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

5.  Synthesis of full length cDNAs from four partially purified oviduct mRNAs.

Authors:  G N Buell; M P Wickens; F Payvar; R T Schimke
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157

6.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

7.  Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors.

Authors:  H Trachsel; B Erni; M H Schreier; T Staehelin
Journal:  J Mol Biol       Date:  1977-11       Impact factor: 5.469

8.  Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

Authors:  M D Biggin; T J Gibson; G F Hong
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

9.  Processing of adenovirus 2-induced proteins.

Authors:  C W Anderson; P R Baum; R F Gesteland
Journal:  J Virol       Date:  1973-08       Impact factor: 5.103

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  49 in total

1.  Tobacco VDL gene encodes a plastid DEAD box RNA helicase and is involved in chloroplast differentiation and plant morphogenesis.

Authors:  Y Wang; G Duby; B Purnelle; M Boutry
Journal:  Plant Cell       Date:  2000-11       Impact factor: 11.277

2.  Selective translational control and nonspecific posttranscriptional regulation of ribosomal protein gene expression during development and regeneration of rat liver.

Authors:  R Aloni; D Peleg; O Meyuhas
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272

3.  Nucleotide sequence of a fission yeast gene encoding the DEAH-box RNA helicase.

Authors:  S B Inoue; H Sakamoto; H Sawa; Y Shimura
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971

4.  ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae.

Authors:  S Blum; S R Schmid; A Pause; P Buser; P Linder; N Sonenberg; H Trachsel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-15       Impact factor: 11.205

5.  An 'equalized cDNA library' by the reassociation of short double-stranded cDNAs.

Authors:  M S Ko
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

6.  Translation initiation factor 4A from Saccharomyces cerevisiae: analysis of residues conserved in the D-E-A-D family of RNA helicases.

Authors:  S R Schmid; P Linder
Journal:  Mol Cell Biol       Date:  1991-07       Impact factor: 4.272

7.  Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12.

Authors:  R G Lloyd; G J Sharples
Journal:  J Bacteriol       Date:  1991-11       Impact factor: 3.490

8.  Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F.

Authors:  F Rozen; I Edery; K Meerovitch; T E Dever; W C Merrick; N Sonenberg
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272

9.  Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12.

Authors:  M Seki; H Miyazawa; S Tada; J Yanagisawa; T Yamaoka; S Hoshino; K Ozawa; T Eki; M Nogami; K Okumura
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

10.  Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system.

Authors:  S Blum; M Mueller; S R Schmid; P Linder; H Trachsel
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205
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