Literature DB >> 6348021

Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae.

L Jahnke, H P Klein.   

Abstract

The effect of oxygen on squalene epoxidase activity in Saccharomyces cerevisiae was investigated. In cells grown in standing cultures, the epoxidase was localized mainly in the "mitochondrial" fraction. Upon aeration, enzyme activity increased and the newly formed enzyme was associated with the "microsomal" fraction. At 0.03% (vol/vol) oxygen, epoxidase levels doubled, whereas the ergosterol level was only slightly increased. Cycloheximide inhibited the increase in epoxidase under these conditions. An apparent Km for oxygen of 0.38% (vol/vol) was determined from a crude particulate preparation for the epoxidase.

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Year:  1983        PMID： 6348021      PMCID： PMC217714          DOI： 10.1128/jb.155.2.488-492.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  On the mechanism of enzymatic cyclization of squalene.

Authors:  T T TCHEN; K BLOCH
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Authors:  H P KLEIN; N R EATON; J C MURPHY
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3.  A quantitative colorimetric assay for squalene.

Authors:  G H ROTHBLAT; D S MARTAK; D KRITCHEVSKY
Journal:  Anal Biochem       Date:  1962-07       Impact factor: 3.365

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Authors:  A A ANDREASEN; T J STIER
Journal:  J Cell Comp Physiol       Date:  1954-06

5.  Comparative properties of 2,3-oxidosqualene-lanosterol cyclase from yeast and liver.

Authors:  I Shechter; F W Sweat; K Bloch
Journal:  Biochim Biophys Acta       Date:  1970-12-16

6.  Involvement of cytochrome P-450 in delta 22-desaturation in ergosterol biosynthesis of yeast.

Authors:  S Hata; T Nishino; M Komori; H Katsuki
Journal:  Biochem Biophys Res Commun       Date:  1981-11-16       Impact factor: 3.575

7.  Subcellular sites involved in lipid synthesis in Saccharomyces cerevisiae.

Authors:  H P Klein; C M Volkmann; M A Leaffer
Journal:  J Bacteriol       Date:  1967-07       Impact factor: 3.490

8.  Improved chamber for the isolation of anaerobic microorganisms.

Authors:  M E Cox; J I Mangels
Journal:  J Clin Microbiol       Date:  1976-07       Impact factor: 5.948

9.  Cellular localization of acetyl-coenzyme A synthetase in yeast.

Authors:  H P Klein; L Jahnke
Journal:  J Bacteriol       Date:  1968-11       Impact factor: 3.490

10.  Oxygen as a factor in eukaryote evolution: some effects of low levels of oxygen on Saccharomyces cerevisiae.

Authors:  L Jahnke; H P Klein
Journal:  Orig Life       Date:  1979-09
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  21 in total

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Authors:  Roger E Summons; Alexander S Bradley; Linda L Jahnke; Jacob R Waldbauer
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2.  Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.

Authors:  Risa Burr; Emerson V Stewart; Wei Shao; Shan Zhao; Hans Kristian Hannibal-Bach; Christer S Ejsing; Peter J Espenshade
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Authors:  K A Donald; R Y Hampton; I B Fritz
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4.  Methyl sterol and cyclopropane fatty acid composition of Methylococcus capsulatus grown at low oxygen tensions.

Authors:  L L Jahnke; P D Nichols
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

5.  Global gene expression analysis of yeast cells during sake brewing.

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Journal:  Appl Environ Microbiol       Date:  2006-09-22       Impact factor: 4.792

6.  Microaerobic steroid biosynthesis and the molecular fossil record of Archean life.

Authors:  Jacob R Waldbauer; Dianne K Newman; Roger E Summons
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-08       Impact factor: 11.205

7.  Characterization of squalene epoxidase activity from the dermatophyte Trichophyton rubrum and its inhibition by terbinafine and other antimycotic agents.

Authors:  B Favre; N S Ryder
Journal:  Antimicrob Agents Chemother       Date:  1996-02       Impact factor: 5.191

Review 8.  Sterol biosynthesis inhibitors: their current status and modes of action.

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Review 9.  Involvement of heme components in sterol metabolism of Saccharomyces cerevisiae.

Authors:  R T Lorenz; L W Parks
Journal:  Lipids       Date:  1991-08       Impact factor: 1.880

10.  Construction of squalene-accumulating Saccharomyces cerevisiae mutants by gene disruption through homologous recombination.

Authors:  N Kamimura; M Hidaka; H Masaki; T Uozumi
Journal:  Appl Microbiol Biotechnol       Date:  1994-11       Impact factor: 4.813
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